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Immunocytochemical localization of heparan sulphate proteoglycan in the rat submandibular gland

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Summary

Heparan sulphate proteoglycan is the predominant proteoglycan synthesized by the parenchymal cells of the rat submandibular gland. A polyclonal antibody was used to localize this proteoglycan in the adult rat submandibular gland. Localization was accomplished by indirect immunoperoxidase cytochemistry at the light and electron microscopic levels. Heparan sulphate proteoglycan was localized in a continuous, linear pattern in the lamina densa of the basement membrane surrounding all of the epithelial components of the gland as well as the basement membrane of the capillaries and small arterioles in the glandular stroma. In addition, heparan sulphate proteoglycan was seen in vesicles and pits along the acinar cell basal plasmalemma adjacent to the basement membrane and in the endoplasmic reticulum and Golgi apparatus of the acinar cells.

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Authors and Affiliations

  1. Department of Morphological Sciences, University of Parana, Curitiba, Brazil

    Waldemiro Gremski

  2. Department of Oral Diagnosis, University of Connecticut Health Center, School of Dental Medicine, 06030, Farmington, CT, USA

    Leslie S. Cutler

Authors
  1. Waldemiro Gremski
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  2. Leslie S. Cutler
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Gremski, W., Cutler, L.S. Immunocytochemical localization of heparan sulphate proteoglycan in the rat submandibular gland. Histochem J 23, 339–344 (1991). https://doi.org/10.1007/BF01042178

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  • DOI: https://doi.org/10.1007/BF01042178

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