Summary
mRNA for the α- and β-subunits of mouse β-N-acetylhexosaminidase were detected in tissue sections of epididymis and testis using3H-labelled antisense RNA probes by hybridizationin situ. There were clear differences in the intensity of the signals given by the two tissues with α-subunit mRNA being more abundant in testis than epididymis, while the opposite was so with β-subunit mRNA. Differences in the isoenzyme patterns of the two tissues are likely to be due, at least in part, to differences in transcription by the Hexa and Hexb genes.
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Angerer, L. M., Cox, K. H. &Angerer, R. C (1987) Demonstration of tissue specific gene expression byin situ hybridization.Methods in Enzymology 152, 649–61.
Beccari, T., Orlacchio, A. &Stirling, J. L. (1988) Identification of β-N-acetylhexosaminidase A in mouse tissues with the fluorigenic substrate 4-methylumbelliferyl-β-N-acetylglucosaminide-6-sulphate.Biochem. J. 252, 617–20.
Bracey, L. T. &Paigen, K. (1987). Changes in translational yield regulate tissue-specific expression of β-glucuronidase.Proc. Natl. Acad. Sci. USA 84, 9020–4.
Conchie, J. &Findlay, J. (1959) Influence of gonadectomy, sex hormones and other factors on the activity of certain glycosidases in the rat and mouse.J. Endocrinol. 18, 132–46.
Conchie, J. &Mann, T. (1957) Glycosidases in mammalian sperm and seminal plasma.Nature 179, 1190–91.
Cook, G. A., Pope, A. M. S. &Stirling, J. L. (1983) β-N-Acetylhexosaminidases ofPhysarum polycephalum: Some characteristics of a new form of the enzyme.J. Gen. Micro. 129, 547–55.
Every, D. &Ashworth, J. M. (1973) The purification and properties of extracellular glycosidases from the cellular slime mouldDictyostelium discoideum.Biochem. J. 133, 37–44.
Ham, J., Thomson, A., Needham, M., Webb, P. &Parker, M. (1988) Characterization of response elements for androgens, glucocorticoids and progestins in mouse mammary tumour virus.Nucleic Acids Research 16, 5263–76.
Jauhiainen, A. &Vanha-Perttula, T. (1966) β-N Acetylglucosaminidase in the reproductive organs and seminal plasma of the bull.J. Reprod. Fert. 76, 239–50.
Majumder, G. C., Lessin, S. &Turkington (1975) Hormonal regulation of isoenzymes of N-acetyl-β-glucosaminidase and β-galactosidase during spermatogenesis in the rat.Endocrinology 96, 890–7.
Neote, B., Bapat.B., Dumbrille-Ross, A., Troxel, C., Schuster, S. M., Mahuran, D. J. &Gravel, R. A. (1988) Characterization of the human HEXB gene encoding lysosomal β-hexaminidase.Genomics 3, 279–86.
Neufeld, E. F. (1989) Natural history and inherited disorders of a lysosomal enzyme β-hexosaminidase.J. Biol. Chem. 264, 10927–30.
Robinson, D. &Stirling, J. L. (1968) N-acetyl-β-glucosaminidases of human spleen.Biochem. J. 107, 321–7.
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Stirling, J.L., Beccari, T., Hoade, J. et al. Analysis of the patterns of expression of mRNAs for the α- and β-subunits of the lysosomal enzyme β-N-acetylhexosaminidase in mouse epididymis and testis. Histochem J 23, 490–494 (1991). https://doi.org/10.1007/BF01041380
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DOI: https://doi.org/10.1007/BF01041380