Summary
We have used quantitative immunoelectronmicroscopy to compare thein situ localization of acid α-glucosidase, lysosomal acid phosphatase, β-hexosaminidase and glucocerebrosidase in intestinal epithelial cells of the human duodenum. Differences between these four lysosomal enzymes were observed with respect to their presence at the apical cell surface. Transport to the apical membrane seems to be a more important intracellular route for lysosomal acid phosphatase and acid α-glucosidase than it is for β-hexosaminidase. The membrane associated lysosomal enzyme glucocerebrosidase is not transported to the microvilli. The studies emphasize that lysosomal enzyme transport pathways are enzyme and cell type specific.
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Willemsen, R., Brünken, R., Sorber, C.W.J. et al. A quantitative immunoelectronmicroscopic study on soluble, membrane-associated and membrane-bound lysosomal enzymes in human intestinal epithelial cells. Histochem J 23, 467–473 (1991). https://doi.org/10.1007/BF01041377
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DOI: https://doi.org/10.1007/BF01041377