Summary
In this paper, data dealing with the sensitivity of autophagy towards partial ATP depletion in isolated rat hepatocytes are reviewed. Partial reduction of intracellular ATP causes: (1) a decrease of proteolytic flux; (2) a decrease in uptake of cytosolic components into the autophagic-lysosomal compartment; (3) either a decrease or no change in the ratio between volume densities of autophagosomes and lysosomes, depending on whether or not the cytosolic phosphate potential is affected; and (4) impairment of the lysosomal proton pump. It is concluded that the consecutive steps of autophagy all respond to relatively small changes of intracellular ATP concentration.
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References
Dunn, W. A., Jr (1990) Studies on the mechanisms of autophagy: formation of the autophagic vacuole.J. Cell Biol. 110, 1923–33.
Grinde, B. &Seglen, P. O. (1980) Differential effects of proteinase inhibitors and amines on the lysosomal and non-lysosomal pathways of protein degradation in isolated rat hepatocytes.Biochim. Biophys. Acta 632, 73–86.
Groen, A. K., Vervoorn, R. C., Wanders, R. J. A., Van Der Meer, R. &Tager, J. M. (1982) An evaluation of the metabolite indicator method for determining the cytosolic phosphate potential in rat liver cells.Biochim. Biophys. Acta 721, 172–7.
Hopgood, M. F., Clark, M. G. &Ballard, F. J. (1977) Inhibition of protein degradation in isolated rat hepatocytes.Biochem. J. 164, 399–407.
Ohkuma, S., Moriyama, Y. &Takano, T. (1982) Identification and characterization of a proton pump on lysosomes by fluorescein isothiocyanate-dextran fluorescence.Proc. Natl. Acad. Sci. USA 79, 2758–62.
Plomp, P. J. A. M., Wolvetang, E. J., Groen, A. K., Meijer, A. J., Gordon, P. B. &Seglen, P. O. (1987) Energy dependence of autophagic protein degradation in isolated rat hepatocytes.Eur. J. Biochem. 164, 197–203.
Plomp, P. J. A. M., Gordon, P. B., Meijer, A. J., Hoyvik, H. &Seglen, P. O. (1989) Energy dependence of different steps in the autophagic-lysosomal pathway.J. Biol. Chem. 264, 6699–704.
Reunanen, H. &Nykanen, P. (1988) A cytochemical study on the effects of energy deprivation on autophagocytosis in Ehrlich ascites tumor cells.Histochemistry 90, 177–84.
Schellens, J. P. M., Vreeling-Sindelárová, H., Plomp, P. J. A. M. &Meijer, A. J. (1988) Hepatic autophagy and intracellular ATP. A morphometric study.Exp. Cell Res. 177, 103–8.
Schellens, J. P. M., Vreeling-sindelárová, H., Plomp, P. J. A. M. &Meijer, A. J. (1990) Cytochemical and morphometric analysis of autophagy in energy depleted rat hepatocytes.Cell Biol. Int. Rep. 14, 805–14.
Schneider, D. L. (1981) ATP-dependent acidification of intact and disrupted lysosomes.J. Biol. Chem. 256, 3858–64.
Schneider, D. L. (1983) ATP-dependent acidification of membrane vesicles isolated from purified rat liver lysosomes.J. Biol. Chem. 258, 1833–8.
Seglen, P. O., Grinde, B. &Solheim, A. E. (1979) Inhibition of the lysosomal pathway of protein degradation in isolated rat hepatocytes.Eur. J. Biochem. 95, 215–25.
Shelburne, J. D., Arstila, A. U. &Trump, B. F. (1973) Studies on cellular autophagocytosis.Am. J. Pathol. 73, 641–70.
Simpson, M. (1953) The release of labeled amino acids from the proteins of rat liver slices.J. Biol. Chem. 201, 143–54.
Steinberg, S. &Vaughan, M. (1956) Observations on intracellular protein catabolism, studied in vitro.Arch. Biochem. Biophys. 65, 93–105.
Woods, H. F., Eggleston, L. V. &Krebs, H. A. (1970) The cause of hepatic accumulation of fructose 1-phosphate on fructose loading.Biochem. J. 119, 501–10.
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Schellens, J.P.M., Meijer, A.J. Energy depletion and autophagy. Cytochemical and biochemical studies in isolated rat hepatocytes. Histochem J 23, 460–466 (1991). https://doi.org/10.1007/BF01041376
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DOI: https://doi.org/10.1007/BF01041376