Synopsis
There is a great deal of evidence to indicate that organs other than the kidney are involved in erythropoietin production. In this paper, it is reported that erythropoietin has been localized with an immunocytochemical method in the granular ducts of the submandibular glands of the rat and mouse.
Similar content being viewed by others
References
Bloom, G. D., Carlsöö, B., Danielsson, A., Marklund, S. &Stigbrand, T. (1974). Peroxidase and amylase activity of the guinea pig submandibular gland during the secretory cycle.Histochemie 38, 271–80.
Busutill, R. W., Roh, B. L. &Fisher, J. W. (1971). The cytological localization of erythropoietin in the human kidney using the fluorescent antibody technique.Proc. Soc. exp. Biol. Med. 137, 327–30.
Byyny, R. L., Orth, D. N. &Cohen, S. (1972). Radioimmunoassay of epidermal growth factor.Endocrinol. 90, 1261–6.
Byyny, R. L., Orth, D. N., Cohen, S. &Doyne, E. S. (1974). Epidermal growth factor: effects of androgens and adrenergic agents.Endocrinol. 95, 776–82.
Dagneaux, P. G. L. C. K., Goudsmit, R. &Krijnen, H. W. (1968). Investigations on an immunoassay of erythropoietin.Ann. N.Y. Acad. Sci. 149, 294–7.
Dorado, M., Langton, A. A. &Espada, J. (1971). Studies on production and some properties of antiserum against erythropoietin.Biochem. Med. 5, 195–204.
Espada, J. &Gutnisky, A. (1970). Purification of human urinary erythropoietin.Acta physiol. latinoamer. 20, 122–9.
Fisher, J. W. (1968). Erythropoietin.Ann. N.Y. Acad. Sci. 149, 1–583.
Goldwasser, E. &Kung, C. K. H. (1971). Purification of erythropoietin.Proc. natn. Acad. Sci. USA 68, 697–8.
Gordon, A. S. &Zanjani, E. D. (1970). The renal crythropoietic factor (erythrogenin) and erythropoietin (ESF). In:Hemopoietic Cellular Proliferation (ed. F. Stohlman Jr), p. 97 New York: Grune & Stratton.
Graham, C. J. &Karnovsky, M. J. (1966). The early stages of absorption of injected horseradish peroxidase in the proximal tubules of mouse kidney: Ultrastructural cytochemistry by a new technique.J. Histochem. Cytochem. 14, 291–302.
Gregory, H. (1975). Isolation and structure of urogastrone and its relationship to epidermal growth factor.Nature 257, 325–7.
Gresik, E. &Barka, T. (1977). Immunocytochemical localization of epidermal growth factor in mouse submandibular gland.J. Histochem. Cytochem. 25, 1027–35.
Hardisty, R. M. &Weatherall, D. J. (1974).Blood and its disorders. p. 91 Oxford: Blackwell.
Jacobson, L. O., Goldwasser, E., Plzak, L. &Fried, W. (1957) Studies on erythropoiesis. IV. Reticulocyte response of hypophysectomized and polycythemic rodents to erythropoietin.Proc. Soc. exp. Biol. Med. 94, 243–9.
Levi-Montalcini, R. &Angeletti, P. U. (1968). Nerve growth factor.Physiol. Rev. 48, 534–69.
Nakane, P. K. &Pierce, G. B. (1967). Enzyme-labeled antibodies for the light and electron microscopic localization of tissue antigens.J. Cell Biol. 33, 307–18.
Oliver, W. J. &Gross, F. (1967). Effect of testosterone and duct ligation on submaxillary renin-like principle.Am. J. Physiol. 213, 341–6.
Petrali, J. P., Hinton, D. M., Moriarty, G. C. &Sternberger, L. A. (1974). The unlabeled antibody enzyme method of immunocytochemistry. Quantitative comparison of sensitivities with and without peroxidase-antiperoxidase complex.J. Histochem. Cytochem. 22, 782–801.
Pitts, A. &Williams, E. E. (1974). Destruction of endogenous peroxidase activity in order to locate cellular antigens peroxidase-labeled antibodies.J. Histochem. Cytochem. 22, 51–4.
Schachter, M. (1969). Kallikreins and kinins.Physiol. Rev. 49, 509–47.
Schooley, J. C. &Mahlmann, L. J. (1972). Erythropoietin production in the anephric rat. I. Relationship between nephrectomy, time of hypoxic exposure, and erythropoietin production.Blood 39, 31–8.
Schooley, J. C. &Mahlmann, L. J. (1974). Extrarenal erythropoetin production in the lead-poisoned rat.Blood 43, 425–8.
Schwab, M. E., Stockel, K. &Thoenen, H. (1976). Immunocytochemical localization of nerve growth factor (NGF) in the submandibular gland of adult mice by light and electron microscopy.Cell Tiss. Res. 169, 289–99.
Sternberger, L. A. &Petrali, J. P. (1977). The unlabeled antibody enzyme method. Attempted use of peroxidase-conjugated antigen as the third layer in the technique.J. Histochem. Cytochem. 25, 1036–42.
Ugarte, C. A. A., Soriano, G., Giglio, J. M. &Bozzini, C. E. (1976). Effect of parasympathetic denervation or duct ligation of rat submandibular glands on extrarenal erythropoietin production.J. Endocrinol. 70, 309–10.
Van Noorden, S., Heitz, P. &Pearse, A. G. E. (1977). Mouse epidermal growth factor: light and electron microscopical localization by immunocytochemical staining.Histochemistry 52, 329–40.
Wintrobe, M. M. (1974).Clinical Hematology. 7th Edn. p. 180. Philadelphia: Lea & Febiger.
Zangheri, E. O., Lopez, O. I., Parisii, I. M. &Silva, J. C. (1972). Dose response curve to a single dose of erythropoietin in polycythemic mice.Acta physiol. latinoamer,22, 181–6.
Zangheri, E. O., Fava-de-Moraes, F., Lopez, O. I. &Marias, I. (1973). The role of submandibular glands on extrarenal erythropoietin production.Experientia 29, 706–7.
Zangheri, E. O. (1977). Reduced extrarenal erythropoietin production in rats treated with isoproterenol.Exp. Hemar. 5, 211–4.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Fava-De-Moraes, F., Zangheri, E.O. & Doine, A.I. Immunohistochemical localization of erythropoietin in the rat and mouse submandibular gland. Histochem J 11, 97–102 (1979). https://doi.org/10.1007/BF01041268
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF01041268