Summary
A sulfonated polysulfone membrane reactor was used forin situ regeneration and retention of coenzymes NADP (H) using the xylose reductase ofCandida pelliculosa coupled with oxidoreductase system ofMethanobacterium sp. in the reduction of xylose to xylitol with hydrogen gas. The membrane could almost completely reject the permeation of NADP (H) (92 and 97%), F420 (97%) and the required enzymes (100%), but not reject for the permeation of xylitol (product). After 4-h reaction for the production of xylitol from xylose (93% yield), although 25% NADP (H) initially added was lost its activity due to unavoidable degradation, the membrane could reject the permeation of the remaining NADP (H) and F420 at the level of 90 and 95%, respectively.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Chambers, R. P., Ford, J. R., Allender, J. H., Baricos, W. H. and Cohen, W. (1974). Continuous processing with cofactor requiring enzyme: coenzyme retention and regeneration. In: Enzyme Engineering, Pye, E. K. and Wingard, L. B. eds vol. 2, pp. 195–202, Newyork: Plenum
Eguchi, S. Y., Nakata, H., Nishio, N. and Nagai, S. (1984). Appl. Microbiol. Biotechnol. 20, 213–217.
Jaenchen, R., Schőnheit, P., and Thauer, R. K. (1984). Arch. Microbiol. 137, 362–365.
Kitpreechavanich, V., Hayashi, M., Nishio, N. and Nagai, S. (1984). Biotech. Lett. 10, 651–656.
Klingenberg, M. (1974). Nicotinamide Adenine Dinucleotides: Spectrophotometric and Fluorometric Methods. In: Method of Enzymatic Analysis, Bergmeyer, H. U., ed vol. 4, pp. 2050–2055, Newyork and London: Academic Press.
Muramatsu, M., Urabe, I., Yamada, Y. and Okada, H. (1977). Eur. J. Biochem., 80, 111–117.
Nelson, M. J. K., Brown, D. P. and Ferry, J. G. (1984). Biochem. Biophys. Res. Comm., 128, 775–781.
Nishio, N., Eguchi, S. Y., Ryuno, K., Kawashima, H. and Nagai, S. (1983). J. Ferment. Technol. 61, 437–441.
Tzeng, S. F., Wolfe, R. S. and Bryant, M. P. (1975). J. Bacteriol. 121, 184–191.
Wong, C. H. and Whitesides, G. M. (1981). J. Am. Chem. Soc. 103, 4809–4899.
Wichman, R., Wandrey, C., Bűckman, A. F. and Kula, M. R. (1981). Biotech. Bioeng. 23, 2789–2802.
Wykes, J. R., Dunnil, P. and Lilly, M. D. (1975). Biotech. Bioeng. 17, 51–68.
Yamazaki, Y., Maeda, N. and Suzuki, H. (1976). Biotech. Bioeng. 18, 1761–1775.
Yoshitake, J., Shimamura, M., Ishizaki, H. and Irie, Y. (1976). Agric. Biol. Chem. 40, 1493–1503.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Kitpreechavanich, V., Nishio, N., Hayashi, M. et al. Regeneration and retention of NADP (H) for xylitol production in an ionized membrane reactor. Biotechnol Lett 7, 657–662 (1985). https://doi.org/10.1007/BF01040204
Issue Date:
DOI: https://doi.org/10.1007/BF01040204