Summary
2-methyl-oxazolinones and thio-thiazolidones representative of aromatic and aliphatic amino acids were hydrolysed by α-chymotrypsin and subtilisin. The parametric ratio kcat/Km, correlated with the enantiomeric enrichment of the reaction product, indicates that thio-thiazolidones are converted to free amino acids by enzyme with the higher degree of stereospecificity.
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Pugnière, M., Kraicsovits, F., Coletti-Previero, MA. et al. Enzymatic hydrolysis of asymmetric heterocyclic amino acid derivatives. Biotechnol Lett 7, 641–646 (1985). https://doi.org/10.1007/BF01040201
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DOI: https://doi.org/10.1007/BF01040201