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Partial characterization of cell wall from a flocculent strain ofKluyveromyces marxianus

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Summary

Cell walls from aKluyveromyces marxianus either non flocculent or flocculent strain were isolated and analysed for protein, carbohydrates and phosphate content. Alkaline extract of proteins were analysed by SDS-PAGE. The results revealed a higher protein content in the cell walls from the flocculent strain. Electrophoresis of the cell wall proteins of the flocculent strain showed an extra peptide with an apparent molecular weight of 37 KDa which is absent fom non-flocculent cells. The involvement of this protein in cell adhesion during flocculation is discussed.

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Authors and Affiliations

  1. Centro de Engenharia Química [INIC], Universidade do Porto, R. dos Bragas, 4000, Porto, Portugal

    Teixeira J., Gama F. M. & Mota M.

  2. Laboratório de Bioquimica, Instituto de Ciencias Biomédicas Abel Salazar, Universidade do Porto, 4000, Porto, Portugal

    Gonçalves M. H. & Moradas-Ferreira P.

Authors
  1. Teixeira J.
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  2. Gonçalves M. H.
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  3. Gama F. M.
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  4. Moradas-Ferreira P.
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  5. Mota M.
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Teixeira, J., Gonçalves, M.H., Gama, F.M. et al. Partial characterization of cell wall from a flocculent strain ofKluyveromyces marxianus . Biotechnol Lett 11, 579–582 (1989). https://doi.org/10.1007/BF01040039

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