Abstract
A novel restriction fragment length polymorphism (RFLP) in inbred rats was revealed by Southern blot analysis with a clone arbitrarily chosen from a rat genomic library as a probe. A clone named α403 showed interstrain variations in the length of theEcoRI andHindIII fragments. TheEcoRI fragments were either 0.7 or 3 kb, those ofHindIII were either 4.5 or 5 kb, and three types were identified as combinations of those fragments in 20 inbred rat strains. These types segregated in backcross progeny as codominant alleles. The locus for the RFLP was thus namedA403. Analysis of linkage between the RFLP locus and 13 other loci reveal that theA403 locus was closely linked to theCs-1 locus (15 ± 5.2%), which belongs to rat linkage group XIII.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Adams, M., Baverstock, P. R., Watts, C. H. S., and Gutman, G. A. (1984). Enzyme marker in inbred rat strains: Genetics of new markers and strain profiles.Biochem. Genet. 22661.
Baker, D., and White, R. (1982). More base pair change polymorphisms at site containing CpG.Cytogenet. Cell Genet. 32253.
Baker, D., Schafer, M., and White, R. (1984). Restriction sites containing CpG show a higher frequency of polymorphism in human DNA.Cell 36131.
Bender, K., Nagel, M., and Gunther, E. (1982). Es-6, a further polymorphic esterase in the rat.Biochem. Genet. 20221.
Bender, K., Bissort, S., Kuhn, A., Nagel, M., and Gunther, E. (1985). Genetic variation of an acid phosphatase (Acp-1) in the laboratory rat: Possible homology with mouseAP-1 and humanACP2.Biochem. Genet. 241.
Davisson, M. T., Roderick, T. H., Doolittle, D. P., Hillyard, A. L., and Guidi, J. N. (1990).Mus musculus (mouse) nuclear genes. In O'Brien, S. J. (ed.),Genetic Maps; Locus Maps of Complex Genomes 5th ed., Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, pp. 4.3–4.35.
Denhardt, D. T. (1966). A membrane-filter technique for the detection of complementary DNA.Biochem. Biophys. Res. Commun. 23641.
Devereux, J., Haeberli, P., and Smithies, O. (1984). A comprehensive set of sequence analysis program for the VAX.Nucleic Acids Res. 12387.
Donis-Keller, H., Green, P., Helms, C., Cartihour, S., Weiffenbach, B., Stephens, K., Keith, T. P., Bowden, D. W., Smith, D. R., Lander, E. S., Botstein, D., Akots, G., Rediker, K. S., Gravius, T., Brown, V. A., Rising, M. B., Parker, C., Powers, J. A., Watt, D. E., Kauffman, E. R., Bricker, A., Phipps, P., Miller-Kahle, H., Fulton, T. R., Ng, S., Schumm, J. W., Braman, J. C., Knowlton, R. G., Backer, D., Crooks, S. M., Lincoln, S. E., Daly, M. J., and Abrahamson, J. (1987). A genetic linkage map of the human genome.Cell 51319.
Feinberg, A. P., and Vogelstein, B. (1983). A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity.Anal. Biochem. 1326.
Gasser, D. L. (1982). Seminal vesicle protein in rats: A gene in the fourth linkage group determining electrophoretic variants.Biochem. Genet. 661.
Gill, T. J., III, Smith, G. J., Wissler, S. R., and Kunz, H. W. (1989). The rat as an experimental animal.Science 245269.
Hattori, M., and Sakaki, Y. (1986). Dideoxy sequencing method using denatured plasmid templates.Anal. Biochem. 152232.
Hedrich, H. J. (1990). Linkage map. In Hedrich, H. J. (ed.),Genetic Monitoring of Inbred Strains of Rats Gustav Fischer Verlag, Stuttgart, pp. 405–409.
Hughes, S. H., Payvar, F., Spector, D., Schimke, R. T., Robinson, H. L., Payne, G. S., Bishop, J., and Varmus, H. E. (1979). Heterogeneity of genetics loci in chickens: Analysis of endogenous viral and nonviral genes by cleavage of DNA with restriction endonuclease.Cell 18347.
Kanehisa, M. (1984). Use of statistical criteria for screening potential homology in nucleic acids sequences.Nucleic Acids Res. 12203.
Kendall, P. B. (1985). Glucose phosphate dehydrogenase polymorphism and the genetics of linkage group II in the Norway rat (Rattus norvegicus).Lab. Anim. 19169.
Kobayashi, E., Kunieda, T., Ikadai, H., Imamichi, T., and Matsumoto, K. (1992). Genetic profiles of 11 inbred rat strains at 25 biochemical loci and five RFLP loci.Lab. Anim. Sci. 4286.
Kunieda, T., Ikadai, H., Matsui, M., Nomura, N., Imamichi, T., and Ishizaki, R. (1989). Novel restriction fragment length polymorphism of the growth hormone gene in inbred rats.Biochem. Genet. 27591.
Kunieda, T., Kobayashi, E., Ikadai, H., Imamichi, T., Nomura, N., and Ishizaki, R. (1990). Restriction fragment length polymorphisms detected in N-ras related sequences of rats and their linkage analyses.Biochem. Genet. 28631.
Levan, G., Szpirer, J., Szpirer, C., Klinga, K., Hanson, C., and Islam, M. Q. (1991). The gene map of the Norway rat (Rattus norvegicus) and comparative mapping with mouse and man.Genomics 10699.
Matsumoto, K. (1980). New polymorphism of esterase 7 and esterase 8 in inbred strains of rats: Tissue expression and linkage studies.Biochem. Genet. 18879.
Mizusawa, S., Nishimura, S., and Seela, F. (1986). Improvement of the dideoxy chain termination method of DNA sequencing by use of deoxy-7-deazaguanosine triphosphate in place of dGTP.Nucleic Acids Res. 141319.
Nikaido, H., Yamada, J., and Kondo, Y. (1982). Male urinary protein-1 (Mup-1) in the rat:Mup-1 assigned to linkage group II.J. Hered. 73119.
Pearson, P. L., Bakker, E., and Flavell, R. A. (1982). Consideration in designing an efficient strategy for localizing unique sequence DNA fragments to human chromosomes.Cytogenet. Cell Genet. 32308.
Sanger, F., Nicklen, S., and Coulson, A. T. (1977). DNA sequencing with chain-terminating inhibitors.Proc. Natl. Acad. Sci. USA 745463.
Siracusa, R. D., Silan, C. M., Justice, M. J., Mercer, J. A., Bauskin, A. R., Ben-Neriah, Y., Duboule, D., Hastie, N. D., Copeland, N. G., and Jenkins, N. A. (1990). A molecular genetic linkage map of mouse chromosome 2.Genomics 6491.
Southern, E. M. (1975). Detection of specific sequences among DNA fragments separated by gel electrophoresis.J. Mol. Biol. 98503.
White, R., Leppert, M., Bishop, D. T., Barker, D., Berkowitz, J., Brown, C., Callahan, P., Holm, T., and Jerominski, L. (1985). Construction of linkage maps with DNA markers for human chromosome.Nature 313101.
Yamada, J., Nikaido, H., and Kondo, Y. (1981). Genetic studies of RBC catalase in the rat (Rattus norvegicus).Jpn. J. Genet. 56447.
Yamada, T., Mori, M., Hamada, S., Serikawa, T., and Yamada, J. (1989). Assignment of tremulous body mutant gene zitter (zi) to linkage group IV of the rat (Rattus norvegicus).J. Hered. 80383.
Author information
Authors and Affiliations
Additional information
This work was supported by a Grant-in-Aid for Scientific Research from the Ministry of Education, Science, and Culture of Japan.
Rights and permissions
About this article
Cite this article
Takahashi, M., Yokota, H., Ayusawa, D. et al. A restriction fragment length polymorphism (RFLP) locus of rat (Rattus norvegicus) linked to theCs-1 locus of rat linkage group XIII. Biochem Genet 30, 537–544 (1992). https://doi.org/10.1007/BF01037592
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF01037592