Summary
The onset of senescence, i.e. decrease of growth rate followed by cellular death, is prevented when inhibitors of mitochondrial function (ethidiumbromide, streptomycin, tiamulin) are present in the culture medium. If mycelia are transferred to a medium not containing one of these substances, senescence occurs after the usual time interval (30 d at 26° C). Inhibitors of cytoplasmic protein synthesis such as emetine and cycloheximide have no effect in preventing senescence.
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References
Bandlow, W., Kaudewitz, F.: Action of ethidium bromide on mitochondrial DNA in the petite-negative yeastSchizosaccharomyces pombe. Molec. gen. Genet.131, 333–338 (1974)
Bertrand, H., McDougall, K.J., Pittenger, T.H.: Somatic cell variation during uninterrupted growth ofNeurospora crassa in continuous growth tubes. J. gen. Microbiol.50, 337–350 (1968)
Bonanchang, D.U., Scavizzi, M.R., Chabbert, Y.A.: Elimination by ethidium bromide of antibiotic resistance in enterobacteria and staphylococci. J. gen. Microbiol.54, 417–425 (1969)
Davies, J., Jones, D.S., Khorana, H.G.: A further study of misreading of codons induced by streptomycin and neomycin using ribopolynucleotides containing two nucleotides in alternating sequence as templates. J. molec. Biol.18, 48–57 (1966)
Ephrussi, B., Hootinguer, H., Chimens, A.M.: Action de l'acriflavine sur les levures. I. La mutation “peptite colonie”. Ann. Inst. Pasteur76, 351–367 (1949)
Esser, K.:Podospora anserina. In: R.C. King (ed.), Handbook of genetics, Vol. I, pp. 531–551. New York: Plenum Press 1974
Esser, K., Keller, W.: Genes inhibiting senescence in the ascomycetePodospora anserina. Molec. gen. Genet.144, 107–110 (1976)
Esser, K., Tudzynski, P.: The prevention of senescence in the ascomycetePodospora anserina by the antibiotic Tiamulin. Nature (Lond.)265, 454–456 (1977)
Gorini, L., Kataja, E.: Phenotypic repair by streptomycin of defective genotypes inEscherichia coli. Proc. nat. Acad. Sci. (Wash.)51, 487–493 (1964)
Högenauer, G.: The mode of action of pleuromutilin as compared to chloramphenicol. In: J. Drews and F.E. Hahn (edts.), Topics in infectious diseases, Vol. 1. Drug Receptor Interactions in Antimicrobial Chemotherapy Symp. Vienna 1974, pp. 235–244. Berlin-Heidelberg-New York: Springer 1975
Marcou, D.: Notion de longévité et nature cytoplasmique du déterminant de la sénescence chez quelques champignons. Ann. Sci. natur. Botan.11, 653–764 (1961)
Schatz, G., Mason, T.L.: The biosynthesis of mitochondrial proteins. Ann. Rev. Biochem.43, 51–87 (1974)
Smith, J.R., Rubenstein, I.: Cytoplasmic inheritance of the timing of “senescence” inPodospora anserina. J. gen. Microbiol.76, 297–304 (1973)
Waring, M.J.: Drugs which affect the structure and function of DNA. Nature (Lond.)219, 1320–1325 (1968)
Zimmermann, R.A., Rosset, R., Gorini, L.: Nature of phenotypic masking exhibition by drug dependent streptomycin A mutants ofE. coli. J. molec. Biol.57, 403–422 (1971)
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Communicated by F. Kaudewitz
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Tudzynski, P., Esser, K. Inhibitors of mitochondrial function prevent senescence in the ascomycetePodospora anserina . Molec. Gen. Genet. 153, 111–113 (1977). https://doi.org/10.1007/BF01036003
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DOI: https://doi.org/10.1007/BF01036003