Summary
Using an in vitro preparation for protein synthesis, we have studied the effect of the ribosomal protein S1 fromEscherichia coli on the synthesis of the coat protein of the RNA-containing phages Qβ and MS2, on that of an “early” and a ⌈ate” enzyme encoded by the DNA containing phage T7, and on that of anthranilate synthetase, an enzyme encoded by the bacterial tryptophan operon. Our results indicated that for the synthesis of these five proteins the presence of S1 is required. From these results we conclude that S1 is an essential protein for the translation of bacterial and bacteriophage messenger RNA.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Bonner, M., Laskey, A.: A film detection method for tritium labelled proteins and nucleic acids in polyacrylamide gels. Europ. J. Biochem.46, 83–88 (1974)
Hagen, F., Young, E.T.: Regulation of synthesis of bacteriophage T7 lysozyme mRNA. Virology55, 231–241 (1973)
Herrlich, P., Schweiger, M.: RNA polymerase synthesisin vitro directed by Ty phage DNA. Molec. gen. Genet.10, 31–35 (1971)
Ito, J., Cox, E.C., Yanofski, C.: Anthranilate synthetase, an enzyme specified by the tryptophan operon ofEscherichia coli: purification and characterization of component I. J. Bact.97, 725–733 (1969)
Kamen, R., Kondo, M., Romer, W., Weissmann, C.: Reconstitution of Qβ replicase lacking subunit α with protein synthesis interference factor i. Europ. J. Biochem.31, 44–51 (1972)
Maizel, J.V.: Methods in virology, vol. V, p. 179 (K. Maramorosch and H. Koprowski, eds.). New York: Academic Press 1971
Pouwels, P.H., Van Rotterdam, J.:In vitro synthesis of enzymes of the tryptophan operon ofEscherichia coli. Proc. nat. Acad. Sci. (Wash.)69, 1786–1790 (1972)
Schweiger, M., Gold, L.M.: Bacteriophage T4 DNA-dependentin vitro synthesis of lysozyme. Proc. nat. Acad. Sci. (Wash.)63, 1351–1358 (1969)
Szer, W., Hermoso, J.M., Leffler, S.: Ribosomal protein and polypeptide chain initiation in bacteria. Proc. nat. Acad. Sci. (Wash.)72, 2325–2329 (1975)
Tal, M., Aviram, M., Kanarek, A., Weiss, A.: Polyuridilic acid binding and translation byEscherichia coli ribosomes: stimulation by protein I, inhibition by aurintricarboxylic acid. Biochim. biophys. Acta (Amst.)281, 381–392 (1972)
Van Dieijen, G., Van Knippenberg, P.H., Van Duin, J.: The specific role of ribosomal protein S1 in the recognition of native phage RNA. Europ. J. Biochem.64, 511–518 (1976)
Van Dieijen, G., Van der Laken, C.J., Van Knippenberg, P.H., Van Duin, J.: Function ofEscherichia coli ribosomal protein S1 in translation of natural and synthetic messenger RNA. J. molec. Biol.93, 351–366 (1975)
Author information
Authors and Affiliations
Additional information
Communicated by H.G. Wittmann
Rights and permissions
About this article
Cite this article
van Dieijen, G., van Knippenberg, P.H., van Duin, J. et al. The effect of the ribosomal protein S1 fromEscherichia coli on the synthesis in vitro of bacterial-, DNA phage- and RNA phage proteins. Molec. Gen. Genet. 153, 75–80 (1977). https://doi.org/10.1007/BF01035998
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF01035998