Summary
Polyacrylamide gel electrophoresis of the 30S ribosomal proteins derived from six streptomycin resistant strains indicates that each mutation alters the same ribosomal protein (str-r protein). Preliminary data utilizing SDS gels indicates that thestr-r protein has a molecular weight between 10,000 and 20,000 daltons. No significant differences could be detected between the molecular weight of thestr-r protein when it is derived either from a sensitive or from a resistant strain, including those derived from strains carrying multisite mutations of different genetic size. We have estimated the size of the multisitestr-r mutations to be less than 30 base pairs. Two factor crosses withstr-r markers in the trans position demonstrate recombination frequencies expected of closely linked, intragenic markers although cotransfer frequencies, of these same markers from the cis position, are very low. It is concluded that the cotransfer frequencies represent a marker effect and possible explanations are discussed. A reinterpretation of the genetic map of the pneumococcalstr-r locus is presented.
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Communicated by L. Lerman
Most of the material is taken from a thesis (ALR) submitted in partial fulfillment of the requirements for the degree of Doctor of Philosophy in the School of Graduate Studies, of the State University of New York, Upstate Medical Center
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Rosenthal, A.L., Rotheim, M.B. A comparison of the genetic and physical size of the streptomycin resistance locus inPneumococcus . Molec. Gen. Genet. 153, 61–73 (1977). https://doi.org/10.1007/BF01035997
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DOI: https://doi.org/10.1007/BF01035997