Summary
The Host Factor required for in vitro coliphage Qβ RNA replication, a heat-stable RNA binding protein present in uninfectedEscherichia coli, has been detected by both immunological and functional tests inAcinetobacter calcoaceticus, Klebsiella pneumoniae, Pseudomonas aeruginosa andPseudomonas putida. It was not detectable by these criteria inBacillus stearothermophilus, Bacillus subtilis, Caulobacter crescentus, Micrococcus lysodeikticus, Rhodopseudomonas capsulata orSaccharomyces cerevisiae. InEscherichia coli the Host Factor protein has been shown to be associated with ribosomes. It is demostrated here that this association is specific for the 30S ribosomal subunit.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Blumenthal, T., Landers, T.A., Weber, K.: Bacteriphage Qβ replicase contains the protein biosynthesis elongation factors EF-Tu and EF-Ts. Proc. nat. Acad. Sci. (Wash.)69, 1313–1317 (1972)
Brodie, A.F.: Preparation and solubilization of particles: bacterial. Methods Enzymol.5, 51–61 (1962)
Carmichael, G.G., Weber, K., Niveleau, A., Wahba, A.J.: The host factor required for RNA phage Qβ RNA replicationin vitro. Intracellular location, quantitation, and purification by poly(A)-cellulose chromatography. J. biol. Chem.250, 3607–3612 (1975)
DuBow, M.S., Blumenthal, T.: Host factor for coliphage Qβ RNA replication is present inPseudomonas putida. Molec. gen. Genet.141, 113–119 (1975)
Franze de Fernandez, M.T., Eoyang, L., August, J.T.: Factor fraction required for the synthesis of bacteriophage Qβ RNA. Nature (Lond.)219, 588–590 (1968)
Franze de Fernandez, M.T., Hayward, W.S., August, J.T.: Bacterial proteins required for replication of phage Qβ ribonucleic acid. Purification and properties of host factor I, a ribonucleic acid-binding protein. J. biol. Chem.247, 824–831 (1972)
Kamen, R.: A new method for the purification of Qβ RNA-dependent RNA polymerase. Biochim. biophys. Acta (Amst.)262, 88–100 (1972)
Kamen, R.: Structure and function of the Qβ RNA replicase. In: RNA phages, (ed. by N.D. Zinder), pp. 203–234. New York: Cold Spring Harbor Laboratory 1975
Kamen, R., Kondo, M., Römer, W., Weissmann, C.: Reconstitution of Qβ replicase lacking subunit α with protein-synthesis interference factor i. Europ. J. Biochem.31, 44–51 (1972)
Milstein, C., Brownlee, G.G., Cartwright, E.M., Jarvis, J.M., Proudfoot, N.J.: Sequence analysis of immunoglobulin light chain messenger RNA. Nature (Lond.)252, 354–359 (1974)
Nishizuka, Y., Lipmann, F., Lucas-Lenard, J.: Isolation and properties of theEscherichia coli amino acid polymerizing enzymes. Methods Enzymol.12, 708–721 (1968)
Proudfoot, N.J., Brownlee, G.G.: 3′ non-coding region sequences in eukaryotic messenger RNA. Nature (Lond.)263, 211–214 (1976)
Senear, A.W., Steitz, J.A.: Site-specific interaction of Qβ host factor and ribosomal protein S1 with Qβ and R17 bacteriophage RNAs. J. biol. Chem.251, 1902–1912 (1976)
Shapiro, L., Franze de Fernandez, M.T., August, J.T.: Resolution of two factors required in the Qβ-RNA polymerase reaction. Nature (Lond.)220, 478–480 (1968)
Shine, J., Dalgarno, L.: Terminal-sequence analysis of bacterial ribosomal RNA; correlation between the 3′-terminal polypyrimidine sequence of 16S RNA and translational specificity of the ribosome. Europ. J. Biochem.57, 221–230 (1975)
Sojka, G.A., Gest, H.: Integration of energy conversion and biosynthesis in the photosynthetic bacteriumRhodopseudomonas capsulata. Proc. nat. Acad. Sci. (Wash.)61, 1486–1493 (1968)
Steitz, J.A., Sprague, K.U., Steege, D.A., Yuan, R.C., Laughrea, M., Moore, P.B., Wahba, A.J.: RNA·RNA and protein·RNA interactions during the initiation of protein synthesis. In: Symposium on protein-nucleic acid sequences (ed. by H. Vogel). Columbia University. In press (1976)
Yamamoto, K.R., Alberts, B.M., Benzinger, R., lawhorne, L., Treiber, g.: Rapid bacteriophage sedimentation in the presence of polyethylene glycol and its application to large scale virus purification. Virology40, 734–744 (1970)
Author information
Authors and Affiliations
Additional information
Communicated by W. Gilbert
Rights and permissions
About this article
Cite this article
DuBow, M.S., Ryan, T., Young, R.A. et al. Host factor for coliphage Qβ RNA replication: Presence in procaryotes and association with the 30S ribosomal subunit inEscherichia coli . Molec. Gen. Genet. 153, 39–43 (1977). https://doi.org/10.1007/BF01035994
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF01035994