Skip to main content
SpringerLink
Log in

Characterization of glycerol nonutilizing and protoperithecial mutants ofNeurospora

  • Published:
Molecular and General Genetics MGG Aims and scope Submit manuscript

Summary

Mutants defective in polyol metabolism and/or in protoperithecial development were selected inNeurospora tetrasperma, a species in which protoperithecial development occurs at nonpermissively high temperature if certain polyols are used in lieu of sucrose as carbon source. Mutants selected for nonutilization of one of the four polyols tested, glycerol, mannitol, sorbitol, or xylitol, were usually found to be nonutilizers of the other three polyols as well. Mutants blocked at various stages of protoperithecial development complemented pairwise to produce more advanced developmental stages, usually mature protoperithecia and, when of opposite mating type, mature perithecia. About one-third of the mutants manifested both polyol auxotrophy and defective protoperithecial development upon initial isolation, but protoperithecial defectiveness in such mutants usually showed erratic segregation in crosses and/or instability to repeated vegetative transfer, whereas polyol auxotrophy usually did not and was, therefore, studied further. Two glycerol nonutilizing strains were introgressed intoN. crassa to facilitate genetic analysis. One,glp-4, lacked both inducible and constitutive glycerol kinase and mapped to linkage group VI, betweenad-1 andrib-1; the other,glp-5, lacked glyceraldehyde kinase and mapped to linkage group I, proximal toad-9. Another mutant,gly-u(234), has been reported by other investigators to lack inducible glycerol kinase but to map to linkage group I, distal toad-9.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Bergmeyer, H.U., Holz, G., Kauder, E.M., Mollering, H., Wieland, O.: Kristallisierte glycerokinase ausCandida mycoderma. Biochem. Z.333, 471–480 (1961)

    Google Scholar 

  • Bischoff, W.L.: Genetic control of soluble NAD-dependent sorbitol dehydrogenase inDrosophila melanogaster. Biochem. Genet.14, 1019–1039 (1976)

    Google Scholar 

  • Bublitz, C., Kennedy, E.P.: Synthesis of phosphatides in isolated mitochondria III. The enzymatic phosphorylation of glycerol. J. biol. Chem.211, 951–961 (1954)

    Google Scholar 

  • Bublitz, C., Wieland, O.: Glycerokinase. In: Methods in enzymology (S.P. Colowick and N.O. Kaplan, eds.), Vol. 5, pp. 354–361. New York: Academic Press 1962

    Google Scholar 

  • Courtright, J.B.: Intracellular localization and properties of glycerokinase and glycerosphosphate dehydrogenase inNeurospora crassa. Arch. Biochem. Biophys.167, 21–33 (1975a)

    Google Scholar 

  • Courtright, J.B.: Differential rates of synthesis of glycerokinase and glycerophosphate dehydrogenase inNeurospora crassa during induction. Arch. Biochem. Biophys.167, 34–44 (1975b)

    Google Scholar 

  • Courtright, J.B.: Characteristics of a glycerol utilization mutant ofNeurospora crassa. J. Bact.124, 497–502 (1975c)

    Google Scholar 

  • Denor, P., Courtright, J.B.: Requirement for specific carbon sources in the low temperature induction of glycerol kinase inNeurospora crassa. FEBS Letters48, 314–316 (1974)

    Google Scholar 

  • Denor, P., Courtright, J.B.: Characteristics of mutants ofNeurospora crassa defective in glycerol utilization. Abstr. Ann. Meeting, Amer. Soc. Microbiol., p. 111 (1975)

  • Esser, K., Straub, J.: Genetische Untersuchungen anSordaria macrospora Auersw. Kompensation und Induktion bei genbedingten Entwicklungsdefekten. Z. Vererbungsl.89, 729–746 (1958)

    Google Scholar 

  • Fitzgerald, P.H.: Genetic and epigenetic factors controlling female sterility inNeurospora crassa. Heredity18, 41–62 (1963)

    Google Scholar 

  • Freedberg, W.B., Lin, E.C.C.: Three kinds of controls affecting the expression of theglp regulon inEscherichia coli. J. Bact.115, 816–823 (1973)

    Google Scholar 

  • Fuller, R.C., Tatum, E.L.: Inositol phospholipid in Neurospora and its relationship to morphology. Amer. J. Bot.43, 361–365 (1956)

    Google Scholar 

  • Gancedo, C., Gancedo, J.M., Sols, A.: Glycerol metabolism in yeasts. Pathways of utilization and production. Europ. J. Biochem.5, 165–172 (1968)

    Google Scholar 

  • Gornall, A.G., Bardawill, C.T., David, M.M.: Determination of serum proteins by means of the biuret reaction. J. biol. Chem.117, 751–766 (1949)

    Google Scholar 

  • Heinz, F., Lamprecht, W.: Anreicherung und Charakterisierung einer Triosekinase aus Leber. Zur Biochemie des Fructosestoffwechsel III. Hoppe-Seylers Z. physiol. Chem.324, 88–100 (1961)

    Google Scholar 

  • Hers, H.G.: Triokinase. In: Methods in enzymology (S.P. Colowick and N.O. Kaplan, eds.), Vol. 5, pp. 362–364 New York: Academic Press 1962

    Google Scholar 

  • Holm, K., Nilheden, E., Kolmark, H.G.: Genetic and enzymatic analysis of a glycerol kinase deficient mutant inNeurospora crassa. Molec. gen. Genet.144, 11–15 (1976)

    Google Scholar 

  • Horecker, B.L.: Pentoses and Pentitols. The role of pentitols and other polyols in evolutionary development. In: Pentoses and pentitols (B.L. Horecker, K. Lang and Y. Takagi, eds.), pp. 5–25, Berlin-Heidelberg-New York: Springer 1969

    Google Scholar 

  • Howe, H.B.: Sources of error in genetic analysis inNeurospora tetrasperma. Genetics50, 181–189 (1964)

    Google Scholar 

  • Howe, H.B., Haysman, P.: Linkage group establishment inNeurospora tetrasperma by interspecific hybridization withN. crassa. Genetics54, 293–302 (1966)

    Google Scholar 

  • Howe, H.B., Johnson, T.E.: Phenotypic diversity among alleles at theper-1 locus ofNeurospora crassa. Genetics82, 595–603 (1976)

    Google Scholar 

  • Howe, H.B., Viswanath-Reddy, M., Bennett, S.N.: Polyol nonutilizing mutants ofNeurospora tetrasperma. Genetics80, 43 (1975)

    Google Scholar 

  • Itoh, T., Morishita, K.: Effect of cytoplasm on protoperithecium formation inNeurospora crassa. Jap. J. Genet.46, 7–15 (1971)

    Google Scholar 

  • Lin, E.C.C.: Glycerol dissimilation and its regulation in bacteria. Ann. Rev. Microbiol.30, 535–578 (1976)

    Google Scholar 

  • Lowry, O.H., Passonneau, J.V.: A flexible system of enzymatic analysis. New York: Academic Press 1972

    Google Scholar 

  • Metzenberg, R.L., Ahlgren, S.K.: Behavior ofNeurospora tetrasperma mating type genes introgressed intoN. crassa. Canad. J. Genet. Cytol.15, 571–576 (1973)

    Google Scholar 

  • Mindich, L.: Membrane synthesis inBacillus I. Isolation and properties of strains bearing mutation in glycerol metabolism. J. molec. Biol.49, 415–432 (1970)

    Google Scholar 

  • Mylyk, O.M., Threlkeld, S.F.H.: A genetic study of female sterility inNeurospora crassa. Genet. Res. Camb.24, 91–102 (1974)

    Google Scholar 

  • Nasrallah, J.B., Srb, A.M.: Genetically related protein variants specifically associated with fruiting body maturation in Neurospora. Proc. nat. Acad. Sci. (Wash.)70, 1891–1893 (1973)

    Google Scholar 

  • Nilheden, E., Holm, K., Kolmark, H.G.: Glycerol nonutilizing mutants inNeurospora crassa. Hereditas (Lund)79, 239–250 (1975)

    Google Scholar 

  • North, M.J.: Cold-induced increase of glycerol kinase inNeurospora crassa. FEBS Letters35, 67–70 (1973)

    Google Scholar 

  • North, M.J.: Cold-induced increase of glycerol kinase activity inNeurospora crassa: rapid inactivation of the enzymein vivo. J. Bact.120, 741–747 (1974)

    Google Scholar 

  • North, M.J.: Influence of the carbon source on glycerol kinase activity inNeurospora crassa. Biochim. biophys. Acta (Amst.)422, 316–325 (1976)

    Google Scholar 

  • Perkins, D.D., Newmeyer, D., Taylor, C.W., Bennett, D.C.: New markers and map sequences inNeurospora crassa, with a description of mapping by duplication coverage, and of multiple translocation stocks for testing linkage. Genetica40, 247–278 (1969)

    Google Scholar 

  • Rothschild, H., Suskind, S.P.: Protoperithecia inNeurospora crassa: technique for studying their development. Science154, 1356–1357 (1966)

    Google Scholar 

  • Sillero, M.A.G., Sillero, A., Sols, A.: Enzymes involved in fructose metabolism in liver and the glyceraldehyde metabolic crossroads. Europ. J. Biochem.10, 345–350 (1969)

    Google Scholar 

  • Srb, A.M., Basl, M., Bobst, M., Leary, J.V.: Mutations inNeurospora crassa affecting ascus and ascospore development. J. Heredity64, 242–246 (1973)

    Google Scholar 

  • Stadler, D.R.: A map of linkage group VI ofNeurospora crassa. Genetics41, 528–543 (1956)

    Google Scholar 

  • Tan, S.T., Ho, C.C.: A gene controlling the early development of protoperithecium inNeurospora crassa. Molec. gen. Genet.107, 158–161 (1970)

    Google Scholar 

  • Thorner, J.W., Pauius, H.: Glycerol and glycerate kinases. In: The enzymes (P.D. Boyer, gen, ed.), Vol. 3, pp. 487–508. New York: Academic Press 1973

    Google Scholar 

  • Toews, C.J.: Evidence for the metabolism of glycerol by skeletal muscle and the presence of a muscle nicotinamide adenine dinucleotide phosphate-dependent glycerol dehydrogenase. Biochem. J.98, 27c-29c (1966)

    Google Scholar 

  • Viswanath-Reddy, M., Turian, G.: Temperature-induced synchronous differentiation of ascogonia in Neurospora. Experientia (Basel)28, 99–100 (1972)

    Google Scholar 

  • Viswanath-Reddy, M., Turian, G.: Physiological changes during protoperithecial differentiation inNeurospora tetrasperma. Physiol. Plant.35, 166–174 (1975)

    Google Scholar 

  • Westergaard, M., Mitchell, H.K.: Neurospora V. A synthetic medium favouring sexual reproduction. Amer. J. Bot.34, 573–577 (1947)

    Google Scholar 

  • Woodward, V.W., DeZeeuw, J.R., Srb, A.M.: The separation and isolation of particular biochemical mutants of Neurospora by differential germination of conidia followed by filtration and selective plating. Proc. nat. Acad. Sci. (Wash.)40, 192–200 (1954)

    Google Scholar 

  • Yamada, M.: Studies on fat metabolism in germinating castor beans IV. Glycerol metabolism. Scientific papers. College of General Education. Tokyo University. Vol. 10, pp. 283–323 (1960)

Download references

Author information

Authors and Affiliations

  1. Department of Microbiology, University of Georgia, 30602, Athens, Georgia, USA

    M. Viswanath-Reddy, Sara N. Bennett & H. Branch Howe Jr.

Authors
  1. M. Viswanath-Reddy
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Sara N. Bennett
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. H. Branch Howe Jr.
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Communicated by F. Kaudewitz

Rights and permissions

Reprints and permissions

About this article

Cite this article

Viswanath-Reddy, M., Bennett, S.N. & Howe, H.B. Characterization of glycerol nonutilizing and protoperithecial mutants ofNeurospora . Molec. Gen. Genet. 153, 29–38 (1977). https://doi.org/10.1007/BF01035993

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01035993

Keywords

Search

Navigation