Summary
We investigated the influence of aminoacidless treatments applied prior and after UV irradiation
on survival, dimer excision, postirradiation DNA degradation, DNA synthesis and sedimentation profiles of parental DNA ofE. coli B/r Hcr+ cells. In dependence on the treatment applied, the fluence 50 J/m2 yielded distinctly different fractions of survivors within 0,03–85%. In all cases dimers were completely excised. The rate of DNA degradation was similar during a 30–40 min period after UV during which the bulk of dimers was excised. Degradation ceased, however, earlier in the prestarved cells than in exponentially growing ones; it was prolonged by aminoacidless postincubation. Sedimentation profiles of parental DNA did not differ during the whole period of dimer excision. In
cells DNA synthesis was not restored for several hours after addition of amino acids. In
cells addition of amino acids resulted in a fast resumption of DNA synthesis. We conclude that removal of dimers and repair of gaps were similar in all cases. We believe that aminoacidless treatments influence production and repair of damage to the sites of DNA replication. The treatment appears to prevent this damage when applied before UV irradiation, but interferes with its restoration when applied after UV irradiation. Consequently, the former treatment increases survival of cells while the latter produces an opposite effects.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Alper, T.: Lethal mutations and cell death. Phys. Med. Biol.8, 365–385 (1963)
Billen, D.: Replication of the bacterial chromosome: location of new initiation sites after irradiation. J. Bact.97, 1169–1175 (1969)
Bonura, T., Smith, K.C., a: Enzymatic production of deoxyribonucleic acid double-strand breaks after ultraviolet irradiation of Escherichia coli K-12. J. Bact.121, 511–517 (1975)
Bonura, T., Smith, K.C., b: Quantitative evidence for enzymatically-induced DNA double-strand breaks as lethal lesions in UV irradiated pool+ and polA 1 strains of E. coli K-12. Photochem. Photobiol.22, 243–248 1975
Bridges, B.A.: Evidence for a further dark repair process in bacteria. Nature (Lond.) New Biol.240, 52–53 (1972)
Carrier, W.L., Setlow, R.B.: Paper strip method for assaying gradient fractions containing radioactive macromolecules. Analyt. Biochem.43, 427–432 (1971)
Cooper, P.K., Hanawalt, P.C.: Heterogeneity of patch size repair replicated DNA in Escherichia coli. J. molec. Biol.67, 1–10 (1972)
Forage, A.J., Gillies, N.E.: Enhancement of damage to Escherichia coli strain B/r after ultraviolet and γ-irradiation. J. gen. Microbiol.59, 329–336 (1969)
Ganesan, A.K., Smith, K.C.: Requirement for protein synthesis in rec− dependent repair of deoxyribonucleic acid in Escherichia coli after ultraviolet and X irradiation. J. Bact.111, 575–585 (1972)
Gillies, N.E.: The use of auxotrophic mutants of study restoration in Escherichia coli B after ultraviolet irradiation. Int. J. Radiat. Biol.3, 379–387 (1961)
Hewitt, R., Billen, D.: Reorientation of chromosome replication after exposure to ultraviolet light of Escherichia coli. J. molec. Biol.13, 40–53 (1965)
Kos, E., Drakuliě, M., Brdar, B.: Response of various strains of Escherichia coli to inhibtion of some biosynthetic processes after ultra-violet irradiation. Nature (Lond.)205, 1125–1126 (1965)
Lark, K.G.: Cellular control of DNA biosynthesis in: Molecular genetics, Part I, pp. 153–206. New York: Acad. Press 1963
Lark, K.G., Repko, T., Hoffman, J.: The effect of amino acid deprivation on subsequent deoxyribonucleic acid replication. Biochim. biophys. Acta (Amst.)76, 9–24 (1963)
McGrath, R.A., Williams, R.W.: Reconstruction in vivo of irradiated Escherichia coli deoxyribonucleic acid; the rejoining of broken pieces. Nature (Lond.)221, 534–535 (1966)
Rupp, W.D., Howard-Flanders, P.: Discontinuities in the DNA synthesized in an excision-defective strain of Escherichia coli following ultraviolet irradiation. J. molec. Biol.31, 291–304 (1968)
Sedliaková, M., Billen, D., Bruns, L.: A study of the relationship between survial and repair synthesis of DNA after ultraviolet light exposure. Photochem. Photobiol.14, 309–317 (1970)
Sedliaková, M., Mašek, F., Bernátová, L.: Relationship between survival and thymine-dimer excision in ultraviolet-irradiated Escherichia coli. Photoshem. Photobiol.14, 597–605 (1971)
Setlow, R.B., in: Regulation of nucleic acid and protein biosynthesis (V.V. Koningsberger and L. Bosch, eds.), pp. 51–62 Amsterdam: Elsevier 1967
Van der Schueren, E., Smith, K.C.: Inhibition of the exrA genedependent branch of the DNA excision repair system in Escherichia coli K-12 by 2,4-dinitrophenol. Photochem. Photobiol.19, 95–102 (1974)
Youngs, D.A., Smith, K.C.: Evidence for the control by exrA and polA genes of two branches of the uvr gene-dependent excision repair pathway in Escherichia coli K-12. J. Bact.116, 175–182 (1973)
Youngs, D.A., Van der Schueren, E., Smith, K.C.: Separate branches of the uvr gene-dependent excision repair process in ultraviolet-irradiated Escherichia coli K-12 cells; their dependence upon growth medium and the polA, recA, recB, and exrA genes. J. Bact.117, 717–725 (1974)
Author information
Authors and Affiliations
Additional information
Communicated by H. Böhme
Rights and permissions
About this article
Cite this article
Sedliaková, M., Prachař, J. & Mašek, F. Dependence of DNA dark repair on protein synthesis inEscherichia coli . Molec. Gen. Genet. 153, 23–27 (1977). https://doi.org/10.1007/BF01035992
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF01035992