Summary
A fifth cytoplasmic mutation (cap r 1) obtained inPodospora anserina is described. In addition to chloramphenicol resistance it confers a strong deficiency in cytochrome aa3 and impairs the germination of ascospores. Genetic analysis shows: 1) strict maternal inheritance of (cap r 1) allele; 2) selection against the (cap r 1) allele as well in sexual crosses as during vegetative growth; 3) complete reversion of this selection by even low concentration of CAP. On the basis of their cytoplasmic inheritance and altered cytochrome spectra the five cytoplasmic mutations are assumed to be mitochondrial. Analysis of crosses between them allows to class them in 3 loci, 2 of which being closely linked.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Adoutte, A., Beisson, J.: Evolution of mixed populations of genetically different mitochondria inParamecium aurelia. Nature (Lond.)235, 393–396 (1972)
Belcour, L.: Cytoplasmic mutations isolated by the use of protoplasts inPodospora anserina. Genet. Res. Cambr.25, 155–161 (1975)
Bertrand, H., Pittenger, T.H.: Cytoplasmic mutants selected from continuously growing cultures ofNeurospora crassa. Genetics61, 643–659 (1969)
Bertrand, H., Pittenger, T.H.: Isolation and classification of extranuclear mutants ofNeurospora crassa. Genetics71, 521–533 (1972)
Birky, C.W., Jr.: Mitochondrial genetics in fungi and ciliates. In: C.W. Birky, Jr., P.S. Perlman, and T.J. Byers (ed) Genetics and biogenesis of mitochondria and chloroplasts. Columbus: The Ohio State University Press 1975
Claisse, M.L., Pere-Aubert, G.A., Clavillier, L.P., Slonimski, P.P.: Méthode d'estimation de la concentration des cytochromes dans les cellules entières de levure. Europ. J. Biochem.30, 469–478 (1970)
Doussière, J., Adoutte, A., Sainsard, A., Ruiz, F., Beisson, J., Vignais, P.: Physiological and genetical analysis of the respiratory chain ofParamecium. In: S. Werner (ed), Genetics and biogenesis of mitochondria and chloroplasts. Amsterdam: North-Holland 1976
Dujon, B., Slonimski, P.P., Weill, L.: Mitochondrial Genetics. IX. A model for recombination and segregation of the mitochondrial genome ofSaccharomyces cerevisiae. Genetics78, 415–437 (1974)
Edwards, D.L., Kwiecinski, F.: Altered mitochondrial respiration in a chromosomal mutant ofNeurospora crassa. J. Bact.116, 610–618 (1973)
Edwards, D.L., Rosenberg, E., Maroney, P.A.: Induction of cyanide-insensitive respiration inNeurospora crassa. J. biol. Chem.249, 3551–3556 (1974)
Grivell, L.A., Netter, P., Borst, P., Slonimski, P.P.: Mitochondrial antibiotic resistance in yeast: Ribosomal mutant resistant to chloramphenicol, erythromycin and spiramycin. Biochim. biophys. Acta (Amst.)312, 358–367 (1973)
Jagow, G. von, Weiss, H., Klingenberg, M.: Comparison of the respiratory chain ofNeurospora crassa wild type and the mutantsmi-1 andmi-3. Europ. J. Biochem.33, 140–157 (1973)
Lambowitz, A.M., Slayman, C.W.: Cyanide-resistant respiration inNeurospora crassa. J. Bact.108, 1087–1906 (1971)
Lambowitz, A.M., Smith, E.W., Slayman, C.W.: Oxidative phosphorylation inNeurospora mitochondria. Studies on wild type,poky, and chloramphenicol-induced wild type. J. biol. Chem.247, 4859–4865 (1972)
Mason, J.R., Turner, G.: Transmission and recombination of extranuclear genes during sexual crosses inAspergillus nidulans. Molec. gen. Genet.143, 93–99 (1975)
Picard, M.: Genetic evidence for a polycistromic unit of transcription in the complex locus “14” inPodospora anserina. I. Genetic and complementation maps. Molec. gen. Genet.111, 35–50 (1971)
Rizet, G.: Les phénomènes de barrage chezPodospora anserina. I. Analyse génétique des barrages entre les souches S et s. Rev. Cytol. Biol. Veget.13, 51–92 (1952)
Rowlands, R.T., Turner, G.: Recombination between the extranuclear genes conferring oligomycin resistance and cold sensitivity inAspergillus nidulans. Molec. gen. Genet.133, 151–161 (1974)
Rowlands, R.T., Turner, G.: Three-marker extra-nuclear mitochondrial crosses inAspergillus nidulans. Molec, gen. Genet.141, 69–79 (1975)
Sainsard, A.: A mitochondrial suppressor of a nuclear gene inParamecium. Nature (Lond.)257, 312–314 (1975)
Sainsard, A., Claisse, M., Balmefresol, M.: A nuclear mutation affecting structure and function of mitochondria inParamecium. Molec. gen. Genet.130, 113–125 (1974)
Tissiéres, A., Mitchell, H.K., Haskins, F.A.: Studies on the respiratory system of thepoky strain ofNeurospora crassa. J. biol. Chem.205, 423–433 (1953)
Turner, G., Rowlands, R.T.: Cytochrome abnormalities and cyanide-resistant respiration in extranuclear mutants ofAspergillus nidulans. J. Bact.125, 389, 397 (1976)
Author information
Authors and Affiliations
Additional information
Communicated by W. Gajewski
Rights and permissions
About this article
Cite this article
Belcour, L., Begel, O. Mitochondrial genes inPodospora anserina: Recombination and linkage. Molec. Gen. Genet. 153, 11–21 (1977). https://doi.org/10.1007/BF01035991
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF01035991