, Volume 41, Issue 5, pp 377–386 | Cite as

Quantitative morphological analysis of erythrocytes by reflection contrast microscopy

  • F. Pera
  • J. Piper
Original Works


In reflection contrast erythrocytes show characteristic interference lines consisting of alternating maxima and minima. The distance between neighboring interference lines corresponds to a difference in cell thickness of about 113 nm. Some visual and graphic methods for quantitative morphological analysis are described; their application is demonstrated using unstained and stained blood smears of normal individuals and patients with hereditary spherocytosis, sickle cell anemia, and thalassemia.

Key words

Reflection contrast Morphology of erythrocytes Stereometry 

Quantitative morphologische Analyse von Erythrozyten mit Hilfe des Reflexionskontrast-Verfahrens


Im Reflexionskontrast-Mikroskop zeigen Erythrozyten charakteristische Interferenzlinien. Zwischen benachbarten Maxima und Minima besteht ein Höhenunterschied von ca. 113 nm. Verschiedene visuelle und zeich-, nerische Methoden der quantitativen morphologischen Analyse der Zellen werden angegeben. Ihre praktische Anwendung wird an ungefärbten und gefärbten Blutausstrichen von Gesunden und von Patienten mit hereditärer Sphärocytose, Sichelzellanämie und Thalassämie demonstriert.


Reflexionskontrast Morphologie der Erythrozyten Stereometrie 


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Copyright information

© Springer-Verlag 1980

Authors and Affiliations

  • F. Pera
    • 1
  • J. Piper
    • 1
  1. 1.Anatomisches Institut der Universität BonnBonn 1Federal Republic of Germany

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