Zusammenfassung
Im Reflexionskontrast-Mikroskop zeigen Erythrozyten charakteristische Interferenzlinien. Zwischen benachbarten Maxima und Minima besteht ein Höhenunterschied von ca. 113 nm. Verschiedene visuelle und zeich-, nerische Methoden der quantitativen morphologischen Analyse der Zellen werden angegeben. Ihre praktische Anwendung wird an ungefärbten und gefärbten Blutausstrichen von Gesunden und von Patienten mit hereditärer Sphärocytose, Sichelzellanämie und Thalassämie demonstriert.
Summary
In reflection contrast erythrocytes show characteristic interference lines consisting of alternating maxima and minima. The distance between neighboring interference lines corresponds to a difference in cell thickness of about 113 nm. Some visual and graphic methods for quantitative morphological analysis are described; their application is demonstrated using unstained and stained blood smears of normal individuals and patients with hereditary spherocytosis, sickle cell anemia, and thalassemia.
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Supported by the Deutsche Forschungsgemeinschaft (Pe 134/2)
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Pera, F., Piper, J. Quantitative morphological analysis of erythrocytes by reflection contrast microscopy. Blut 41, 377–386 (1980). https://doi.org/10.1007/BF01035493
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DOI: https://doi.org/10.1007/BF01035493