Summary
The staphylococcal protein A-gold method was found to be superior to the enzyme- or ferritinlinked antibody techniques for locating capsular antigens on cryosections ofPasteurella haemolytica, and its sensitivity was similar to the enzyme-linked antibody method. The sensitivity of conventionally fixed and embedded material and cryosections of heavily fixed, lightly fixed and unfixed material were shown to be similar under routine laboratory conditions.
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Bendayan, M., Roth, J., Perrelet, A. &Orci, L. (1980) Quantitative immunocytochemical localization of pancreatic secretory proteins in subcellular compartments of the rat acinar cell.J. Histochem. Cytochem. 28, 149–60.
Biberfeld, B., Ghetie, V. &Sjöquist, J. (1975) Demonstration and assaying of IgG antibodies in tissues and on cells by labelled staphylococcal protein A.J. Immunol. Meth. 6, 249–59.
Brands, R., Koninkx-Peeters, R., Slot, J. W. & Geuze, H. J. (1980)Electron Microscopy, Vol. 2, pp. 330–331. Leiden: Seventh European Congress on Electron Microscopy Foundation.
Echlin, P., Skaer, H. L. B., Gardiner, B. O. C., Franks, F. &Asquith M. H. (1977) Polymeric cryoprotectants in the preservation of biological, ultrastructure. II. Physiological effects.J. Microsc. 110, 239–55.
Forsgren, A. &Sjöquist, J. (1966), ‘Protein A’ fromS. aureus: I. Pseudoimmune reaction with human gamma globulin.J. Immunol. 97, 822–7.
Goudswaard J., Van Der Donk, J. A., Noordzi, J. A., Van Dam, R. H. &Vaerman J. P. (1978) Protein A reactivity of various mammalian immunoglobulins.Scand. J. Immunol. 8, 21–8.
Griffiths, G. W. &Jockusch, B. M. (1980) Antibody labelling of thin sections of skeletal muscle with specific antibodies: A comparison of bovine serum albumin (BSA) embedding and ultracryomicrotomy.J. Histochem. Cytochem. 28, 969–78.
Kraehenbuhl, J. P., Racine, L. &Jamieson, J. D. (1977) Immunocytochemical localization of secretory proteins in bovine pancreatic exocrine cells.J. Cell. Biol. 72, 406–23.
Mason, D. Y. &Biberfeld, P. (1980) Technical aspects of lymphoma immunohistology.J. Histochem. Cytochem. 28, 731–45.
Painter, G., Tokuyasu, K. T. &Singer, S. J. (1973) Immunoferritin localisation of intracellular antigens: the use of ultracryotomy to obtain ultrathin sections suitable for direct immunoferritin staining.Proc. natn. Acad. Sci., U.S.A.,70, 1649.
Penn, C. W. &Nagy, L. K (1976) Isolation of a protective non-toxic capsular antigen fromPasteurella multocida, types B and E.Res. Vet. Sci. 20, 90–6.
Roll, F. J., Madri, J. A., Albert, J. &Furthmayr, H. (1980) Codistribution of collagen types IV and AB2 in basement membranes and mesangium of the kidney. An immunoferritin study of ultrathin frozen sections.J. Cell. Biol. 85, 597–616.
Roth, J., Bendayan, M. &Orci, L. (1978) Ultrastructural localization of intracellular antigens by the use of protein A-gold complex.J. Histochem. Cytochem. 26, 1074–81.
Roth, J. &Binder, M. (1978) Colloidal gold ferritin and peroxidase as markers for electron microscopic double labelling lectin techniques.J. Histochem. Cytochem. 26, 163–9.
Sternberger, L. A. (1979)Immunochemistry. 2nd, edn, pp. 133. New York, Chichester: Wiley.
Tokuyasu, K. T. &Singer, S. J. (1976) Improved procedures for immunoferritin labelling of ultrathin frozen sections.J. Cell. Biol. 71, 894–906.
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Beesley, J.E., Orpin, A. & Adlam, C. A comparison of immunoferritin, immunoenzyme and gold-labelled protein A methods for the localization of capsular antigen on frozen thin sections of the bacterium,Pasteurella haemolytica . Histochem J 14, 803–810 (1982). https://doi.org/10.1007/BF01033629
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DOI: https://doi.org/10.1007/BF01033629