Summary
Coupled ligand-colloidal gold complexes were found to provide a convenient approach for the localization by scanning electron microscopy of cell surface membrane antigens and lectin-binding sites on bladder urothelium and for the immunocytochemical identification of urothelial cell populations at different stages of differentiation. The ligands used to probe the membrane were a urothelium-specific rabbit antibody raised to a urothelial membrane-associated antigen (UMA), and two lectins: Concanavalin A (Con A) and peanut agglutinin (PNA). A complex luminal surface distribution pattern was demonstrated by the UMA antigen related to the stage of urothelial cell maturation and differentiation. UMA could be detected on the surface of immature and early differentiating intermediate cells, but was absent from the late differentiation stage, becoming re-expressed as the cells matured and was found in greatest abundance on the terminally differentiated superficial cells. It was absent on cells in benign hyperplasia of the urothelium. Cellular and regional differences in lectin binding to the urothelial cell surface was suggested with Con A receptors localized uniformly over the superficial cells, and PNA receptors confined to linear arrays or occasional clusters over the apical surface but evenly dispersed over the lateral surface of these cells.
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References
Ackerman, G. A. &Freeman, W. H. (1979) Membrane differentiation of developing hemic cells of the bone marrow demonstrated by changes in concanavalin A surface labelling.J. Histochem. Cytochem. 27, 1413–23.
Alroy, J. &Weinstein, R. S. (1980) Intraepithelial asymmetric unit membrane plaques in mammalian urinary bladder.Anat. Rec. 197, 75–83.
Bittiger, H. &Schnebli, H. P. (1976) (editors)Concanavalin A as a Tool, Chichester: Wiley.
Bourguignon, L. Y. W. &Singer, S. J. (1977) Transmembrane interactions and the mechanism of capping of surface receptors by their specific ligands.Proc. natn. Acad. Sci., U.S.A. 74, 5031–5.
Caruthers, J. M. S. &Bonneville, M. A. (1980) Luminal plasma membrane alterations in bladder cancer.Invest. Urol. 17, 364–72.
Freeman, H. J., Lotan, R. &Kim, Y. S. (1980). Application of lectins for detection of goblet cell glycoconjugate differences in proximal and distal colon of the rat.Lab. Invest. 42, 405–12.
Geuze, H. J., Slot, J. W., Van Derley, P. A. &Scheffer, R. C. T. (1981). Use of colloidal gold particles in double-labeling immunoelectron microscopy of ultrathin frozen tissue sections.J. Cell Biol. 89, 653–65.
Goodman, S. L., Hodges, G. M. &Livingston, D. C. (1980) A review of the colloidal gold marker system.Scanning Electron Microscopy II, 133–46.
Goodman, S. L., Hodges, G. M., Trejdosiewicz, L. K. &Livingston, D. C. (1981) Colloidal gold markers and probes for routine application in microscopy.J. Microsc. 123, 201–13.
Heyderman, E. (1979) Immunoperoxidase technique in histopathology: applications, methods and controls.J. clin. Path. 32, 971–78.
Hicks, R. M. (1975) The mammalian urinary bladder: an accommodating organ.Biol. Rev. 50, 215–46.
Hicks, R. M. &Wakefield, J. St. J. (1976). Membrane changes during urothelial hyperplasia and neoplasia.Cancer Res. 36, 2502–7.
Hicks, R. M., Wakefield, J. St. J. &Chowaniec, J. (1975) Evaluation of a new model to detect bladder carcinogens or co-carcinogens; results obtained with saccharin, cyclanate and cyclophosphamide.Chem. Biol. Interact. 11, 225–33.
Hodges, G. M. (1979) The urinary system-bladder. InBiomedical Research Applications of Scanning Electron Microscopy, Vol. 1 (edited byHodges, G. M. andHallowes, R. C.), pp. 307–353, London: Academic Press.
Hodges, G. M., Hicks, R. M. &Spacey, G. D. (1976) Scanning electron microscopy of cell surface changes in methylnitrosurea (MNU)-treated rat bladdersin vivo andin vitro.Differentiation 6, 143–50.
Hodges, G. M., Hicks, R. M. &Spacey, G. D. (1977). Epithelial-stromal interactions in normal and chemical carcinogen-treated adult bladder.Cancer Res. 37, 3720–30.
Horisberger, M. (1981). Colloidal gold: a cytochemical marker for light and flourescent microscopy and for transmission and scanning electron microscopy.Scanning Electron Microscopy III, 9–13.
Kakizoe, T., Komatsu, H., Niijima, T., Kawachi, T. &Sugimura, T. (1980) Increased agglutinability of bladder cells by concanavalin A after administration of carcinogens.Cancer Res. 40, 2006–9.
Kenemans, P., Davina, J. H. M., De Haan, R. W., Van Der Zanden, P., Vooys, G. P., Stolk, J. G. &Stadhouders, A. M. (1981) Cell surface morphology in epithelial malignancy and its precursor lesions.Scanning Electron Microscopy III, 23–36.
Molday, R. S. &Maher, P. (1980). A review of cell surface markers and labelling techniques for scanning electron microscopy.Histochem. J. 12, 273–315.
Newman, R. A., Klein, P. J. &Rudland, P. S. (1979) Binding of peanut lectin to breast epithelium, human carcinomas, and a cultured rat mammary stem cell: use of the lectin as a marker for mammary differentiation.J. natn. Cancer Inst. 63, 1339–46.
Stackpole, C. W. (1978) Topographical differentiation of the cell surface.Prog. Surf. membr. Sci. 12, 1–182.
Sternberger, L. (1979)Immunocytochemistry, 2nd edn. New York: Wiley.
Trejdosiewicz, L. K., Smolira, M. A., Hodges, G. M., Goodman, S. L. &Livingston, D. C. (1981) Cell surface distribution of fibronectin in cultures of fibroblasts and bladder derived epithelium: SEM-immunogold localization compared to immunoperoxidase and immunofluorescence.J. Microsc. 123, 227–36.
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Hodges, G.M., Smolira, M.A. & Trejdosiewicz, L.K. Urothelium-specific antibody and lectin surface mapping of bladder urothelium. Histochem J 14, 755–766 (1982). https://doi.org/10.1007/BF01033625
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DOI: https://doi.org/10.1007/BF01033625