Summary
Friable calli were obtained fromAchillea millefolium L. hypocotyls, in Gamborg B5 medium, supplemented with 1.5mg.1−1 2,4-D / 0.1mg.1−1 Kin, and used for the production of cell suspension cultures in the same liquid medium. The growth pattern of the cultures was determined in permanent light or dark conditions and with different inoculum densities, basal media, growth regulators and sucrose concentrations. Different sources and nitrogen amounts were assayed to study the effect on yarrow cell growth. The conditions found to be optimal for growth of yarrow cell suspension cultures were: 70g (f.w.).1−1 of initial inoculum in Gamborg B5 medium, supplemented with 1.5mg. 1−1 2,4-D / 0.1mg.1−1 Kin, NO3 −/NH4 + (30/lmM), and 2% sucrose, in darkness. In these culture conditions the cell suspensions showed a doubling time of 35–40h.
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Abbreviations
- 2,4-D:
-
dichlorophenoxyacetic acid
- NAA:
-
naphtalenacetic acid
- BA:
-
benzyladenine
- Kin:
-
Kinetin
References
Battat, E.; J. Roken; I. Goldberg 1989, Plant Cell Rep. 7: 652–654.
Carceller, M.; M. Davey; M. Fowler; H. Street 1971, Protoplasma 73: 367–385.
Drapeau, D.; H. Blanch; C. Wilke 1986 Biotechnol. Bioengng. 28: 1555–1563.
Durzan, D.; 1987, In: Cell and Tissue Culture in Foresty. J. Bonga.; D. Durzan, eds. Vol. 1 Martinus Nijhoff Publishers.
Figueiredo, A. C.; J. Schripsema; R. Verpoorte; M. S. Pais 1990, Internatinal Congress on Plant Tissue and Cell Culture, Amsterdam.
Figueiredo, A. C.; P. Fevereiro; J. Cabral; J. Novais 1987, Biotechnol. Lett. 9: 213–218.
Gamborg, O.; R. Miller; K. Ojima 1968, Exp. Cell Res. 50: 151–158.
Kirby E.; T. Leustek; M. Lee 1987, In: Cell and Tissue Culture in Foresty. J. Bonga; D. Durzan, eds. Vol. 1 Martinus Nijhoff Publishers.
Larkin, P. 1976, Planta 128: 213–216.
Lips, S.; M. Soares; J. Kaiser; O. Lewis 1987, In: Inorganic nitrogen metabolism. Ullrich, W.; P. Aparicio; P. Syrett; F. Castillo, eds. Springer Verlag.
Lowry, O.; N. Rosebrough; A. Farr; R. Randall 1951, J. Biol. Chem. 193: 265–275.
McCown, B.; J. Sellmer 1987, In: Cell and Tissue Culture in Foresty. J. Bonga; D. Durzan, eds. Vol. 1 Martinus Nijhoff Publishers.
Murashige, T.; F. Skoog 1962, Physiol. Plant. 15: 473–497.
Phillips, R.; G. Henshaw 1977, J. Exp. Bot. 28: 785–794.
Schripsema, J.; A. Meijer; F Van Iren; H. Hoopen; R. Verpoorte 1990, Plant Cell, Tissue and Organ Culture (accepted for publication).
Schulz, A. 1981, Ph. D. Thesis Technische Universitat. Hannover.
Simon, J.; A. Chadwick; L. Craker 1984, Herbs: An indexed bibliography. Elsevier.
Twaij, H. 1983, Fitoterapia 54: 25–32.
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Figueiredo, A.C.S., Pais, M.S.S. Achillea millefolium (yarrow) cell suspension cultures: Establishment and growth conditions. Biotechnol Lett 13, 63–68 (1991). https://doi.org/10.1007/BF01033519
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DOI: https://doi.org/10.1007/BF01033519