Summary
To assess optimal saccharification performance, α-cellulose and dilute acid pretreated aspen (DAA) wood meal were subjected to various loadings of commercial cellulase and β-D-glucosidase preparations. Fifteen international filter paper units (IFPU)/g cellulose content and 30 IFPU/g cellulose content were required to digest 95% of the available cellulose in α-cellulose and pretreated aspen, respectively. The optimal supplementation ratios, based on Genencor GC 123 cellulase and β-D-glucosidase from Novo SP 188 for the α-cellulose and DAA digestions range from 0.25 to 0.5 and 0.12 to 0.25, respectively.
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Stockton, B.C., Mitchell, D.J., Grohmann, K. et al. Optimumβ-D-glucosidase supplementation of cellulase for efficient conversion of cellulose to glucose. Biotechnol Lett 13, 57–62 (1991). https://doi.org/10.1007/BF01033518
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DOI: https://doi.org/10.1007/BF01033518