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Effect of sucrose concentration on the invertase activity of intact yeast cells (S. cerevisiae)

Summary

The kinetic parame6ers for soluble (Km=7.4mM and, Vmax=2.6 U/mL) and cell wall bound invertase (Km=15.4mM and Vmax=3.2 U/L) were determined. The invertase activity for both forms varied by around 10% against sucrose concentration ranging from 80 g/L to 200 g/L, whereas the transferase activity increased markedly with increasing sucrose concentration.

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References

  • Abrams, E.; Neigeborn, L. and Carlson, M. (1986).Mol. Cell Biol., 6, 3643–3651.

    Google Scholar 

  • Alfani, F.; Cantarella, M.; Cantarella, L. and Vitolo, M. (1988).Annals of the New York Academy of Sciences,542, 346–350.

    Google Scholar 

  • Barman, T.E. (1969). Enzyme handbook, vol II, p. 584, New York, Springer-Verlag.

    Google Scholar 

  • Berggren, B. (1970).Ark. Kemi, 31, 555–563.

    Google Scholar 

  • Combes, D. and Monsan, P. (1981).Carbohydrate Research, 93, 312–316.

    Google Scholar 

  • Godfrey, T. (1983).Immobilized enzymes. In: Industrial enzymology, T. Godfrey and J. Reichelt eds., p. 437, Hong-Kong, Macmillan Publishers Ltd.

    Google Scholar 

  • Hansen, S.A. (1975).J. of Chromatography, 105, 388–390.

    Google Scholar 

  • Morrison, R.T. and Boyd, R.N. (1969).Organic Chemistry, 2nd Ed., p. 1019, Boston, Allyn and Bacon, Inc.

    Google Scholar 

  • Reddy, A.V.; MacColl, R. and Maley, F. (1990).Biochemistry, 29, 2482–2487.

    Google Scholar 

  • Santín, J.L.; Solà, C. and Lema, J.M. (1982).Biotechnol. Bioeng, 24, 2721–2724.

    Google Scholar 

  • Shearwin, K.E. and Winzor, D.J. (1988).Arch. Biochem. Biophys., 260, (2), 532–539.

    Google Scholar 

  • Straathof, A.J.J.; Kieboom, P.G. and Bekkum,H. (1986).Carbohydrate Research, 146, 154–159.

    Google Scholar 

  • Tammi, M.; Ballou, L.; Taylor, A. and Ballou, C.E. (1987).J. Biol. Chem., 262 (9), 4395–4401.

    Google Scholar 

  • Tracey, M.V. (1963).Biochim. Biophys. Acta, 77, 147–149.

    Google Scholar 

  • Vitolo, M. and Almeida Cunha, B.C. (1984).Biotechnol. Bioeng., 26, 811–813.

    Google Scholar 

  • Vitolo, M. and Borzani, W. (1983).Anal. Biochem., 130, 469–470.

    Google Scholar 

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Vitolo, M., Yassuda, M.T. Effect of sucrose concentration on the invertase activity of intact yeast cells (S. cerevisiae). Biotechnol Lett 13, 53–56 (1991). https://doi.org/10.1007/BF01033517

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  • DOI: https://doi.org/10.1007/BF01033517

Keywords

  • Sucrose
  • Cell Wall
  • Organic Chemistry
  • Yeast Cell
  • Bioorganic Chemistry