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Separation of the components of pectolytic complex produced byTubercularia vulgaris in solid state culture

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Summary

Tubercularia vulgaris produces extracellular pectolytic activity in solid state culture using citrus pulp pellets as a carbon source. Five peaks containing hydrolase activities were separated by ion exchange chromatography (DEAE-Sephacel). Two enzymatic forms, PG II and PG III, were purified about 8 and 10 fold, respectively. The molecular weight of PG II was similar to that of PG III (24.000 Da). The two enzymes had similar endo activities on polygalacturonic acid, as determined by comparison of viscosity reduction and reducing groups release.

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Authors and Affiliations

  1. Departamento de Ciências Farmacêuticas, Faculdade de Ciências Farmacêuticas de Ribeirão Preto-USP, Via do Café, s/no-Bairro Monte Alegre-14.049, Ribeirão Preto, São Paulo, Brasil

    M. J. Vieira Fonseca & S. Said

  2. Departamento de Física e Química, Faculdade de Ciências Farmacêuticas de Ribeirão Preto-USP, Via do Café, s/no-Bairro Monte Alegre-14.049, Ribeirão Preto, São Paulo, Brasil

    A. C. Cropanese Spadaro

Authors
  1. M. J. Vieira Fonseca
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  2. A. C. Cropanese Spadaro
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  3. S. Said
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Vieira Fonseca, M.J., Cropanese Spadaro, A.C. & Said, S. Separation of the components of pectolytic complex produced byTubercularia vulgaris in solid state culture. Biotechnol Lett 13, 39–42 (1991). https://doi.org/10.1007/BF01033514

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