Summary
Enzymatic triglyceride synthesis from free fatty acid and glycerol is shown to be catalysed by both 1,3-specific lipase and non-specific lipase. This paper elucidates the mechanism of the reaction, showing that, with a 1,3-specific lipase, the 1,3-diolein enzymatically formed has to isomerise spontaneously to 1,2-diolein in presence of oleic acid, before being transformed to triolein by the enzyme.
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References
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Ergan, F., Trani, M. Effect of lipase specificity on triglyceride synthesis. Biotechnol Lett 13, 19–24 (1991). https://doi.org/10.1007/BF01033510
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DOI: https://doi.org/10.1007/BF01033510