Summary
Eggs of the nematode,Necator americanus, were incubated at 28°C for up to 120h in medium (prepared from mouse faeces) supplemented with 0.4% (w/v) or 5% of either commercial grade or a silica-Amberlite purified fraction of the non-ionic surfactant, Pluronic F-68. Exposure to commercial grade or purified Pluronic at both concentrations delayed egg hatching. However, subsequent larval growth, as assessed by length of larva, buccal capsule, oesophagus and tail, was significantly (P<0.05) increased (maximally following exposure to 5.0% of the purified fraction). No further development occurred in larvae exposed to other Pluronic fractions tested after 48h, irrespective of concentration. These results suggest that Pluronic may be a valuable supplement in media used for the axenic culture of nematode larvae.
Similar content being viewed by others
References
Bentley, P.K., Davis, S.S., Johnson, O.L., Lowe, K.C. and Washington, C. (1989a)J. Pharm. Pharmac. 41, 661–669.
Bentley, P.K., Gates, R.M.C., Lowe, K.C., de Pomerai, D.I. and Walker, J.A.L. (1989b)Biotechnol. Lett. 11, 111–114.
Cawrse, N., de Pomerai, D.I. & Lowe, K.C. (1991)Biomed. Sci. 2, 180–182.
Handa-Corrigan, A., Emery, A.N. and Spier, R.E. (1989)Enzyme Microb. Technol. 11, 230–235.
King, A.T., Davey, M.R., Mellor, I.R., Mulligan, B.J. and Lowe, K.C. (1991)Enzyme Microb. Technol. 13, 148–153.
Murhammer, D.W. and Goochee, C.F. (1988)Bio/Technology. 6, 1411–1418.
Murhammer, D.W. and Goochee, C.F. (1990)Biotechnol. Prog. 6, 142–148.
Sen, H.G. and Seth, D. (1967)Nature 214, 609–610.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Kumar, S., Laouar, L., Pritchard, D.I. et al. Effects of Pluronic F-68 on larval development of the hookworm,Necator americanus . Biotechnol Lett 13, 599–602 (1991). https://doi.org/10.1007/BF01033417
Issue Date:
DOI: https://doi.org/10.1007/BF01033417