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Stability of the lipase immobilized on DEAE-Sephadex for continuous lipid hydrolysis in organic solvent

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Summary

Continuous hydrolysis reaction was carried out in glass-column by using lipase fromCandida rugosa immobilized on DEAE-Sephadex A50. Substrate olive oil dissolved in hydrophobic organic solvent (isooctane) was supplied into the reactor with a concurrent stream of aqueous buffer. Stability of the immobilized enzyme was greatly increased in higher substrate concentration. Glyccrol added in the aqueous stream showed stabilizing effect against the organic solvent; half life was extended from 220 hrs to 450 hrs by 15% glycerol supplemented at 30°C.

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Authors and Affiliations

  1. Department of Biological Science and Engineering, Korea Advanced Institute of Science and Technology, Chongyang, P.O.Box 150, 130-650, Seoul, Korea

    Dongsoo Yang & Joon Shick Rhee

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  1. Dongsoo Yang
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  2. Joon Shick Rhee
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Yang, D., Rhee, J.S. Stability of the lipase immobilized on DEAE-Sephadex for continuous lipid hydrolysis in organic solvent. Biotechnol Lett 13, 553–558 (1991). https://doi.org/10.1007/BF01033408

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  • DOI: https://doi.org/10.1007/BF01033408

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