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Solid state fermentation of straw withNeurospora crassa for CMCase and β-glucosidase production

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Summary

CMCase and β-glucosidase were produced by the mutantNeurospora crassa 40b cultivated on untreated wheat straw in a solid state fermentation. Best enzyme activities were observed when the growth medium was composed of wheat straw mixed with certain mineral solutions at a ratio 1:2 (w/v). A partially purified enzyme preparation showed optimum enzyme activities of CMCase and β-glucosidase at pH 4.0 and 5.0 and temperature 50 and 60°C respectively. The apparent Km values for the same enzymes were 16.8 g/l and 1.03x10−4 M respectively. At optimum growth and enzyme assay conditions yields as high as 586.2 U CMCase and 58.4 U β-glucosidase per gram of straw were obtained.

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Authors and Affiliations

  1. Biology Institute, NRC DEMOCRITOS, Aghia Paraskevi, 15 310, Attiki, Greece

    B. J. Macris & X. Evangelidou

  2. Department of Chemical Engineering, National Technical University of Athens, 42 28th Octobriou Str., 106 82, Athens, Greece

    B. J. Macris & D. Kekos

  3. Department of Science and Technology of Food and Agricultural Products, Agricultural University of Athens, Votanikos, Athens, Greece

    M. Galiotou-Panayotou & P. Sodis

Authors
  1. B. J. Macris
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  2. D. Kekos
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  3. X. Evangelidou
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  4. M. Galiotou-Panayotou
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  5. P. Sodis
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Macris, B.J., Kekos, D., Evangelidou, X. et al. Solid state fermentation of straw withNeurospora crassa for CMCase and β-glucosidase production. Biotechnol Lett 9, 661–664 (1987). https://doi.org/10.1007/BF01033207

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  • DOI: https://doi.org/10.1007/BF01033207

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