Biotechnology Letters

, Volume 9, Issue 9, pp 633–637 | Cite as

Hyperproduction of N-acetylneuraminate lyase by the gene-cloned strain ofEscherichia coli

  • Kazuo Aisaka
  • Shigeaki Tamura
  • Yuko Arai
  • Takayuki Uwajima
Article

Summary

Optimal culture medium for production ofN-acetylneuraminate lyase (NANA lyase) by the gene-cloned strain ofEscherichia coli, E. coli(pNALl), was screened to develop the process for the industrial production of NANA lyase. Out of nutrients tested, corn steep liquor (CSL) and sugarcane molasses were superior nutrient sources for the enzyme production. Hyperproduction (6–8 units/ml-broth) was achieved on the medium consisted of CSL and molasses.

Keywords

Enzyme Corn Organic Chemistry Nutrient Source Sugarcane 

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References

  1. Aisaka, K., and Uwajima, T. (1986). Appl. Environ. Microbiol. 51, 562–565.Google Scholar
  2. Comb, D. G., and Roseman, S. (1962). Methods Enzymol 5, 391–394.Google Scholar
  3. Laemmli, U. K. (1970). Nature. 227, 680–685.Google Scholar
  4. Lowry, O. H., Rosebrough, N. J., Farr, A. L., and Randall, R. J. (1951). J. Biol. Chem. 193, 265–295.Google Scholar
  5. Sugahara, K., Sugimoto, K., Nomura, O., and Usui, T. (1980). Clin. Chim. Acta. 108, 493–498.Google Scholar
  6. Uchida, Y., Tsukada, Y., and Sugimori, T. (1984). J. Biochem. (Tokyo) 96, 507–522.Google Scholar
  7. Uchida, Y., Tsukada, Y., and Sugimori, T. (1985). Agric. Biol. Chem. 49, 181–187.Google Scholar

Copyright information

© Kluwer Academic Publishers 1987

Authors and Affiliations

  • Kazuo Aisaka
    • 1
  • Shigeaki Tamura
    • 2
  • Yuko Arai
    • 2
  • Takayuki Uwajima
    • 1
  1. 1.Tokyo Research LaboratoryKyowa Hakko Kogyo Co.Machida-shi, Tokyo 194Japan
  2. 2.Fuji FactoryKyowa Hakko Kogyo Co.Shizuoka-ken 411Japan

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