Summary
Deactivation ofCandida rugosa lipase was found to be complex. Hydrophobic interaction induced by iso-octane influenced the initial phase of deactivation, and increased the turn-over rate of the intermediate in the transition phase. After urea-treatment the structure of the last phase was not further influenced by thermal treatment, whereas that of initial phase was more sensitive to temperature change. Charge interaction was important in maintaining the structure during the deactivation, and especially anion charge might be a major factor.
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Sohn, H.S., Chung, S.S. & Rhee, J.S. Deactivation ofCandida rugosa lipase. Biotechnol Lett 9, 117–122 (1987). https://doi.org/10.1007/BF01032749
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DOI: https://doi.org/10.1007/BF01032749