Biotechnology Letters

, Volume 9, Issue 2, pp 105–110 | Cite as

Effect of high concentration of nutrients onBacillus thuringiensis cultures

  • J. Arcas
  • O. Yantorno
  • R. Ertola
Article

Summary

Bacterial insecticide production using a strain ofBacillus thuringiensis var. kurstaki was studied in batch culture considering the influence of increasing concentration of components of a glucose — yeast extract — mineral salts medium.

It was found that spore counts were increased from 1.08×1012 spores. 1−1 to 7.36×1012 spores. 1−1 and toxin level from 1.05 mg.ml−1 to 6.85 mg.ml−1, when the concentration of glucose was increased from 8 to 56 (g 1−1), with the corresponding increase in the rest of medium components. Higher concentration of nutrients inhibit either spore count or toxin production.

Preliminary experiments of fed-batch cultures which allows the use of high amounts of nutrients were also carried out. In this case spore counts of 1.2×1013 spores.1−1 were achieved.

Keywords

Glucose Organic Chemistry Yeast Extract Bioorganic Chemistry Batch Culture 

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Copyright information

© Kluwer Academic Publishers 1987

Authors and Affiliations

  • J. Arcas
    • 1
  • O. Yantorno
    • 1
  • R. Ertola
    • 1
  1. 1.Centro de Investigación y Desarrollo en Fermentaciones Industriales Facultad de Ciencias ExactasLa PlataAngentina

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