Effect of high concentration of nutrients onBacillus thuringiensis cultures
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Bacterial insecticide production using a strain ofBacillus thuringiensis var. kurstaki was studied in batch culture considering the influence of increasing concentration of components of a glucose — yeast extract — mineral salts medium.
It was found that spore counts were increased from 1.08×1012 spores. 1−1 to 7.36×1012 spores. 1−1 and toxin level from 1.05 mg.ml−1 to 6.85 mg.ml−1, when the concentration of glucose was increased from 8 to 56 (g 1−1), with the corresponding increase in the rest of medium components. Higher concentration of nutrients inhibit either spore count or toxin production.
Preliminary experiments of fed-batch cultures which allows the use of high amounts of nutrients were also carried out. In this case spore counts of 1.2×1013 spores.1−1 were achieved.
KeywordsGlucose Organic Chemistry Yeast Extract Bioorganic Chemistry Batch Culture
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