Biotechnology Letters

, Volume 9, Issue 2, pp 105–110 | Cite as

Effect of high concentration of nutrients onBacillus thuringiensis cultures

  • J. Arcas
  • O. Yantorno
  • R. Ertola


Bacterial insecticide production using a strain ofBacillus thuringiensis var. kurstaki was studied in batch culture considering the influence of increasing concentration of components of a glucose — yeast extract — mineral salts medium.

It was found that spore counts were increased from 1.08×1012 spores. 1−1 to 7.36×1012 spores. 1−1 and toxin level from 1.05−1 to 6.85−1, when the concentration of glucose was increased from 8 to 56 (g 1−1), with the corresponding increase in the rest of medium components. Higher concentration of nutrients inhibit either spore count or toxin production.

Preliminary experiments of fed-batch cultures which allows the use of high amounts of nutrients were also carried out. In this case spore counts of 1.2×1013 spores.1−1 were achieved.


Glucose Organic Chemistry Yeast Extract Bioorganic Chemistry Batch Culture 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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  1. Andrews R E, Jr, Iandolo J J, Campbell B S, Davidson L I, Bulla E A, Jr (1980) Appl. Environm Microbiol, 40, 897.Google Scholar
  2. Angus T A (1968) World Rev Pest Control, 7, 11.Google Scholar
  3. Arcas J A, Yantorno O M, Arrarás E A, Ertola R J (1984) Biotech Letters 6, 495.Google Scholar
  4. Cooney C L, Wang D I C (1977) Biotech Bioeng, 19, 55.Google Scholar
  5. Drake B and Smythe C, U.S. Patent, 1963: 3,087,865.Google Scholar
  6. Goldberg I, Sneh B, Battat E, Klein D (1980), Biotech Letters, 2, 419.Google Scholar
  7. Holmberg A, Sievanen R (1980). Biotech Bioeng, 22, 1707.Google Scholar
  8. Hyvarinen A, Nikkila E A (1962). Clin Chim Acta, 7, 140.Google Scholar
  9. Murrell W G (1961) In: Meynell G G & Gooder H (eds) Microbial reaction to environment. The 11th Symposium of the Society for General Microbiology, Cambridge, 100.Google Scholar
  10. Norris J R,in The Bacterial Spore, G W Gould and A Hurst, Eds. (Academic, London, 1969).Google Scholar
  11. Pirt J S (1975) In: Principles of Microbe and Cell Cultivation. Blackwell Scientific Publications, London, 147.Google Scholar
  12. Scherrer P, Luthy P, Trumpi B (1973). Appl Microbio, 25, 644.Google Scholar
  13. Srinivasan V R, Fleenor M B, Summers R J (1977). Biotech Bioeng, 19, 153.Google Scholar
  14. Tyrrell D J, Bulla L A, Jr, Andrews R E, Jr, Kramer K J, Davidson L I, Nordin P (1981). J Bacteriol, 145, 1052.Google Scholar
  15. Watson T G (1970). J Gen Microbiol, 64, 91.Google Scholar
  16. Yamamoto T, McLaughlin R E (1981). Biochem Biophys Res Commun, 103, 414.Google Scholar

Copyright information

© Kluwer Academic Publishers 1987

Authors and Affiliations

  • J. Arcas
    • 1
  • O. Yantorno
    • 1
  • R. Ertola
    • 1
  1. 1.Centro de Investigación y Desarrollo en Fermentaciones Industriales Facultad de Ciencias ExactasLa PlataAngentina

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