Summary
Bacterial insecticide production using a strain ofBacillus thuringiensis var. kurstaki was studied in batch culture considering the influence of increasing concentration of components of a glucose — yeast extract — mineral salts medium.
It was found that spore counts were increased from 1.08×1012 spores. 1−1 to 7.36×1012 spores. 1−1 and toxin level from 1.05 mg.ml−1 to 6.85 mg.ml−1, when the concentration of glucose was increased from 8 to 56 (g 1−1), with the corresponding increase in the rest of medium components. Higher concentration of nutrients inhibit either spore count or toxin production.
Preliminary experiments of fed-batch cultures which allows the use of high amounts of nutrients were also carried out. In this case spore counts of 1.2×1013 spores.1−1 were achieved.
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References
Andrews R E, Jr, Iandolo J J, Campbell B S, Davidson L I, Bulla E A, Jr (1980) Appl. Environm Microbiol, 40, 897.
Angus T A (1968) World Rev Pest Control, 7, 11.
Arcas J A, Yantorno O M, Arrarás E A, Ertola R J (1984) Biotech Letters 6, 495.
Cooney C L, Wang D I C (1977) Biotech Bioeng, 19, 55.
Drake B and Smythe C, U.S. Patent, 1963: 3,087,865.
Goldberg I, Sneh B, Battat E, Klein D (1980), Biotech Letters, 2, 419.
Holmberg A, Sievanen R (1980). Biotech Bioeng, 22, 1707.
Hyvarinen A, Nikkila E A (1962). Clin Chim Acta, 7, 140.
Murrell W G (1961) In: Meynell G G & Gooder H (eds) Microbial reaction to environment. The 11th Symposium of the Society for General Microbiology, Cambridge, 100.
Norris J R,in The Bacterial Spore, G W Gould and A Hurst, Eds. (Academic, London, 1969).
Pirt J S (1975) In: Principles of Microbe and Cell Cultivation. Blackwell Scientific Publications, London, 147.
Scherrer P, Luthy P, Trumpi B (1973). Appl Microbio, 25, 644.
Srinivasan V R, Fleenor M B, Summers R J (1977). Biotech Bioeng, 19, 153.
Tyrrell D J, Bulla L A, Jr, Andrews R E, Jr, Kramer K J, Davidson L I, Nordin P (1981). J Bacteriol, 145, 1052.
Watson T G (1970). J Gen Microbiol, 64, 91.
Yamamoto T, McLaughlin R E (1981). Biochem Biophys Res Commun, 103, 414.
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Arcas, J., Yantorno, O. & Ertola, R. Effect of high concentration of nutrients onBacillus thuringiensis cultures. Biotechnol Lett 9, 105–110 (1987). https://doi.org/10.1007/BF01032747
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DOI: https://doi.org/10.1007/BF01032747