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Transformation of Kluyveromyces lactis cells by plasmid DNA does not require alkaline cations

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Summary

A procedure for the transformation ofKluyveromyces lactis based on the Li salt method for introducing plasmid DNA into intact yeast cells is described. Contrary toSaccharomyces cerevisiae, lithium salts are dispensable for inducing competence inK. lactis. 2-Mercaptoethanol, a compound that stimulates transformation inS. cerevisiae, showed an opposite effect. inK. lactis. On the other hand, the presence of PEG 4000 and a heat shock were absolutely required to obtain high transformation efficiency.

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Authors and Affiliations

  1. Industrial Microbiology Branch, Centro Nacional de Investigaciones Cientificas, P. O. Box 6990, La Habana, Cuba

    Hilda M. Garcia, Maria de los Angeles Martinez, Roberto Vazquez & Julio Delgado

Authors
  1. Hilda M. Garcia
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  2. Maria de los Angeles Martinez
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  3. Roberto Vazquez
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  4. Julio Delgado
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Garcia, H.M., Martinez, M.d.l.A., Vazquez, R. et al. Transformation of Kluyveromyces lactis cells by plasmid DNA does not require alkaline cations. Biotechnol Lett 7, 723–726 (1985). https://doi.org/10.1007/BF01032283

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  • DOI: https://doi.org/10.1007/BF01032283

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