Biotechnology Letters

, Volume 7, Issue 10, pp 711–716 | Cite as

Mutagenesis inClostridiumacetobutylicum

  • S. A. Lemmel


Methanesulfonic acid ethyl ester (EMS) is effective in inducing mutants resistant to ampicillin, erythromycin, and butanol (15 g/l). Optimal mutagenesis occurs at 85–90% kill corresponding to a 15 minute exposure to 1.0% (v/v) EMS at 35 C. At optimal conditions, the frequency of resistant mutant CFU/ total CFU plated increases 100–200 fold.


Ester Ethyl Organic Chemistry Optimal Condition Ampicillin 
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Copyright information

© Science and Technology Letters 1985

Authors and Affiliations

  • S. A. Lemmel
    • 1
  1. 1.Moffett Technical CenterCPC InternationalArgoUSA

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