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Biotechnology Letters

, Volume 7, Issue 10, pp 711–716 | Cite as

Mutagenesis inClostridiumacetobutylicum

  • S. A. Lemmel
Article

Summary

Methanesulfonic acid ethyl ester (EMS) is effective in inducing mutants resistant to ampicillin, erythromycin, and butanol (15 g/l). Optimal mutagenesis occurs at 85–90% kill corresponding to a 15 minute exposure to 1.0% (v/v) EMS at 35 C. At optimal conditions, the frequency of resistant mutant CFU/ total CFU plated increases 100–200 fold.

Keywords

Ester Ethyl Organic Chemistry Optimal Condition Ampicillin 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Science and Technology Letters 1985

Authors and Affiliations

  • S. A. Lemmel
    • 1
  1. 1.Moffett Technical CenterCPC InternationalArgoUSA

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