Biotechnology Letters

, Volume 11, Issue 4, pp 249–254 | Cite as

Glucose condensation by glucoamylase in organic solvents

  • Valérie Laroute
  • René-Marc Willemot
Article

Summary

Oligosaccharides were synthesized through the enzymatic condensation of D-glucose by glucoamylase in water-organic mixtures with high concentrations of two of diethylene glycol diethyl ether or triethylene glycol dimethyl ether. The effect of water content on the yield of reaction was studied; maximum yield was obtained with 10% (v/v) of water in the two systems. Kinetics of synthesis and products composition were different with the two solvents. 37% of glucose were condensed by action of glucoamylase from a reaction medium containing 20 g/L of glucose and 90% (v/v) of diethylene glycol diethyl ether.

Keywords

Glucose Glycol Oligosaccharide Diethyl Ether Reaction Medium 

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Copyright information

© Kluwer Academic Publishers 1989

Authors and Affiliations

  • Valérie Laroute
    • 1
  • René-Marc Willemot
    • 1
  1. 1.Département de Génie Biochimique et AlimentaireUA-CNRS 544, Institut National des Sciences AppliquéesTOULOUSE CédexFrance

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