Biotechnology Letters

, Volume 13, Issue 6, pp 427–432 | Cite as

Production of D-lactic acid from 1,2-propanediol byPseudomonas sp. strain TB-135

  • Toshiya Shigeno
  • Tadaatsu Nakahara


A newly isolated bacterium, strain TB-135, produces solely D-lactic acid from 1,2-propanediol (1,2-PD). From taxonomical studies, the strain was concluded to belong to the genusPseudomonas. The optimal conditions for the acid production were found to be at 4% 1,2-PD and 0.2% yeast extract, when the strain produced 21mg/ml of the acid of a high optical purity (over 99% e.e.) after 4days of cultivation. Since it was considered that the acid was produced from (R)-(−)-1,2-PD, the molar yield was estimated to be 86%.


Organic Chemistry Optimal Condition Yeast Extract Bioorganic Chemistry Acid Production 
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  1. Butilin,K.R., and Wince,W.H. (1939).J. Soc. Chem. Ind., 58, 363–366.Google Scholar
  2. Ishii,M., Haràda,T., and Nikuni,J. (1959). Nippon Nogeikagaku Kaishi, 33, 889–893.Google Scholar
  3. Izumi,Y., Matsumura,Y., Tani,Y., and Yamada,H. (1982).Agr. Biol. Chem., 46, 2673–2679.Google Scholar
  4. Kitahara,K. and Suzuki,J. (1963).J. Appl. Microbiol., 9, 59–71.Google Scholar
  5. Krieg,N.R. and Holt,J.G. (1984). Bergey's Manual of Systematic Bacteriology Vol. 1, The Williams and Willkins Co., Baltimore.Google Scholar
  6. Nishio,N., Kawagishi,T., Matsuno,R., and Kamikubo,T. (1978).Agr. Biol. Chem., 42, 1095–1100.Google Scholar
  7. Takao,S., and Tanida,M., (1977). Nippon Nogeikagaku Kaishi, 51, 239–244.Google Scholar
  8. Tanaka,Y., Fujii,K., Tanaka,A., and Fukui,S. (1975).J. Ferment. Technol., 53, 566–568.Google Scholar
  9. Yagi,O., and Yamada,K. (1969).Agr. Biol. Chem., 33, 1587–1593.Google Scholar
  10. Yamada,Y., Nakamura,H.T., Tahara,Y., Oyaizu,H., and Komagata,K. (1982).J. Appl. Microbiol., 28, 7–12.Google Scholar

Copyright information

© Science and Technology Letters 1991

Authors and Affiliations

  • Toshiya Shigeno
    • 1
  • Tadaatsu Nakahara
    • 1
  1. 1.Institute of Applied BiochemistryUniversity of TsukubaTsukuba, IbarakiJapan

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