Summary
ATP photophosphorylation by spinach thylakoid was examined to evaluate its use as an ATP regeneration reaction in biosynthetic reactors that consume ATP. Initial rate of cyclic photophosphorylation mediated by phenazine methosulfate was found to be 218 μmole ATP/h.mg Chlorophyll. This activity was stable for over 3 months at −85°C. When phosphoryl transfer reactions were coupled to cyclic photophosphorylation, ATP was continuously regenerated by thylakoid between 14–24 times in batch reactors.
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Yu, A.H.C., Hosono, K. ATP regeneration by cyclic photophosphorylation using spinach thylakoid. Biotechnol Lett 13, 411–416 (1991). https://doi.org/10.1007/BF01030992
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DOI: https://doi.org/10.1007/BF01030992