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Higher culture pH is preferable for inclusion body formation of recombinant salmon growth hormone inEsherichia coli

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Summary

Higher culture pH of 7.6 was shown to be preferable for the inclusion body formation of salmon growth hormone (SGH) inEscherichia coli harboring a recombinant plasmid. High-level formation of SGH inclusion bodies was achieved at 33°C (pH 7.6). Growth inhibition by soluble SGH was also observed.

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Authors and Affiliations

  1. Tokyo Research Laboratories, Kyowa Hakko Kogyo Co., Ltd., 3-6-6 Asahimachi, 194, Machida, Tokyo, Japan

    Seiji Sugimoto, Yoshiharu Yokoo, Noritaka Hatakeyama, Akira Yotsuji, Sadao Teshiba & Hiroshi Hagino

Authors
  1. Seiji Sugimoto
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  2. Yoshiharu Yokoo
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  3. Noritaka Hatakeyama
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  4. Akira Yotsuji
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  5. Sadao Teshiba
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  6. Hiroshi Hagino
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Sugimoto, S., Yokoo, Y., Hatakeyama, N. et al. Higher culture pH is preferable for inclusion body formation of recombinant salmon growth hormone inEsherichia coli . Biotechnol Lett 13, 385–388 (1991). https://doi.org/10.1007/BF01030987

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  • DOI: https://doi.org/10.1007/BF01030987

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