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Activity of NAD-dependent glucose-6-phosphate dehydrogenase in yeast-like organisms

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Summary

From tested yeast-like organisms, onlyGeotrichum candidum showed the same activity of glucose-6-phosphate dehydrogenase with both NAD+ and NADP+. i. e. 0.017–0.019 μmol NADH/min. mg dry weight of cell free extracts. Omission of Mg++ in the reaction mixture did not influence the activity of the enzyme in the presence of NAD+. Cell free extracts ofEndomyces magnusii showed only low activity of this enzyme and the ratio of its activity in the presence of NAD+ and NADP+, respectively, varied in individual cultures.Rhodotorula glutinis showed only an NADP+-dependent activity.

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Authors and Affiliations

  1. Department of Biochemistry and Microbiology, Institute of Chemical Technology, Tachnicka 5, 166 28, Prague 6, Czechoslovakia

    Ludmila Ŝilhánková, Sona Novotná & Blanka Králová

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  1. Ludmila Ŝilhánková
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  2. Sona Novotná
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  3. Blanka Králová
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Ŝilhánková, L., Novotná, S. & Králová, B. Activity of NAD-dependent glucose-6-phosphate dehydrogenase in yeast-like organisms. Biotechnol Lett 14, 67–70 (1992). https://doi.org/10.1007/BF01030916

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  • DOI: https://doi.org/10.1007/BF01030916

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