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L-Malic acid production by polyacrylamide gel entrapped Pichia membranaefaciens

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Summary

The production of L-malic acid from fumaric acid has been achieved byPichia membraneafaciens cells entrapped in a polyacrylamide gel lattice. The reaction rate was found to be 0.15 mmoles/h/g of immobilized cells. The optimum pH for fumarase activity of immobilized cells was stable after repeated uses it increased after storing the gel pellets at 5°C. A good yield of L-malic acid production (up to 3.77 g/l) was also observed in wine added with Na fumarate.

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Authors and Affiliations

  1. Istituto di Microbiologia lattiero-casearia, Facoltà di Agraria, Università di Perugia, 06100, Perugia, Italy

    J. Rossi & F. Clementi

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  1. J. Rossi
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  2. F. Clementi
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Rossi, J., Clementi, F. L-Malic acid production by polyacrylamide gel entrapped Pichia membranaefaciens. Biotechnol Lett 7, 329–334 (1985). https://doi.org/10.1007/BF01030280

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