Zusammenfassung
Es wurde ein in vitro-System entwickelt, in dem die Tendenz von Rattenerythrozyten (RE) gemessen wurde, entweder nach einer in vivo-Alterung oder einer in vitro-Oberflächenveränderung durch homologe peritoneale Makrophagen phagozytiert zu werden. Wurden „alte“, dichtere RE in Fraktionen auf Basis ihrer Dichte aufgetrennt, so war die Phagozytose von „alten“ RE wesentlich ausgeprägter als jene von „jüngeren“ Zellen. Die Neuraminidase-Behandlung von RE führte zu einer hochgradigen Phagozytose im Vergleich zu den Normalkontrollen der gesamten Zellpopulation oder „alten“ Zellen. Eine Galaktose-Oxydase-Behandlung von neuraminidasebehandelten RE brachte keinen Schutz gegenüber den Makrophagen. Im Gegensatz dazu führte die Behandlung von RE mit Neuraminidase, die an Sepharose-4B festgebunden ist, zwar zu einer Entfernung bis zu ∼25% der gesamten membrangebundenen Neuraminsäure, ergab jedochkeine über den Kontrollwerten liegende Phagozytose. Daraus folgt, da\ man bei der Interpretation von Experimenten mit Neuraminidase sehr vorsichtig sein mu\, wenn das Enzym dafür verwendet werden soll, künstlich „gealterte“ RE zu erzeugen.
Summary
An in vitro system was developed to test the propensity of rat erythrocytes (RBC) toward phagocytosis by homologous peritoneal macrophages after in vivo aging or after in vitro surface modification. “Old” RBC obtained from erythrocyte populations separated into fractions on the basis of their density were found to be phagocytosed to a significantly greater extent than cells obtained from “young” cell fractions. Neuraminidase treatment of RBC resulted in extensive phagocytosis in comparison to control cells representing the whole population or “old” cells. Galactose oxidase treatment of neuraminidase treated RBC afforded no protection from uptake by macrophages. In contrast, treatment of RBC with neuraminidase immobilized on Sepharose 4B, leading to removal of up to ∼25% of the total membrane sialic acid, did not lead to phagocytosis above control values. These results indicate that extreme caution is necessary in the interpretation of experiments in which neuraminidase is utilized to produce simulated “aged” RBC.
References
Ashwell G, Morell AG (1974) The role of surface carbohydrate in the hepatic recognition and transport of circulating glycoproteins. Adv Enzymol 41: 99–128
Bell W, Levy G, Williams R, Aminoff, D (1976) Effect of galactose oxidase, with and without prior sialidase treatment, on the viability of erythrocytes in circulation. Proc Natl Acad Sci USA 74: 4205–4209
Corfield A, Beau J-M, Schauer R (1978) Desialylation of glycoconjugates using immobilized Vibrio cholerae neuraminidase preparation, properties, and use of the bound enzyme. Hoppe Seyler's Z Physiol Chem 359: 1335–1342
Jancik J, Schauer R (1974) Sialic acid — A determinant of the life-time of rabbit erythrocytes. Hoppe Seylers Z Physiol Chem 355: 395–400
Kay MMB (1975) Mechanism of removal of senescent cells by human macrophages in situ. Proc Natl Acad Sci USA 72: 3521–3525
Kuester J, Schauer R (1980) A radioactive in vitro assay for the recognition and phagocytosis of neuraminidase-treated erythrocytes by macrophages. Hoppe Seylers Z Physiol Chem 361:291
Lueben G., Sedlacek HH, Seiler FR (1976) Quantitative experiments on the cell membrane binding of neuraminidase. Behring Inst Mitt 59: 30–37
Luner SJ, Szklarek D, Knox RJ, Seaman GVF, Josefowicz JY, Ware BR (1977) Red cell charge is not a function of cell age. Nature 269: 719–721
Murphy J (1973) Influence of temperature and method of centrifugation on the separation of erythrocytes. J Lab Clin Med 82: 334–341
Nordt FJ, Knox RJ, Seaman GVF (1978) Red cell aging. II. Anomalous electrophoretic properties of neuraminidase treated human erythrocytes. J Cell Physiol 97: 209–220
Schauer R (1978) Characterization of sialic acids. Methods Enzymol 50: C64–89
Seaman GVF, Knox RJ, Nordt FJ, Regan DH (1977) Red cell aging. I. Surface charge density and sialic acid content of density fractionated human erythrocytes. Blood 50: 1001–1011
Warren L (1959) The thiobarbituric acid assay of sialic acids. J Biol Chem 234: 1971–1975
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Nordt, F.J., Franco, M., Corfield, A. et al. The efficacy of neuraminidase treatment in studies on red cell aging. Blut 42, 95–98 (1981). https://doi.org/10.1007/BF01030031
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01030031