Summary
The volume distribution curves of rat thrombocytes show a significant rise of the modal volume between days 5 and 8 after birth. No clear evidence for two distinct thrombocyte populations was obtained by computer analysis of the volume distribution curves. However, the increase of the modal volume of the platelets correlates with the production of a new erythrocyte population. In addition, the platelet concentration in the blood increases significantly during this time. These changes could indicate the production of a second thrombocyte population. The mean electrophoretic mobility of rat thrombocytes decreases only slightly during the first 3 weeks after birth, while it increases for erythrocytes significantly during the same period.
Zusammenfassung
Die Volumenverteilung von Rattenthrombozyten zeigt zwischen dem 5. und dem 8. Tag nach Geburt eine deutliche Zunahme im Modalvolumen. Bei rechnerischer Analyse der Kurven konnten keine deutlichen Anhaltspunkte für zwei diskrete Thrombozytenpopulationen gefunden werden. Da sich jedoch der Anstieg des Modalvolumens mit dem Beginn der Produktion einer neuen Erythrozytenpopulation deckt, und außerdem die Thrombozytenkonzentration im Blut in dieser Zeit deutlich ansteigt, können diese Veränderungen Ausdruck der Produktion einer zweiten Thrombozytenpopulation sein. Die mittlere elektrophoretische Beweglichkeit der Rattenthrombozyten fällt in den ersten drei Wochen nach Geburt nur geringfügig ab, während die der Erythrozyten im gleichen Zeitraum signifikant anstiegt.
Similar content being viewed by others
Literatur
Blunt MH, Huismann THJ (1975) The haemoglobins of sheep. In: Blunt MH (ed) The blood of sheep. Springer, Berlin Heidelberg New York, pp 155–183
Blanchet J-P (1976) Developmental changes of chick erythrocyte membrane antigens. Exp Cell Res 102: 1–8
Blanchet J-P, Samarut J, Nigon V (1976) Chick erythrocyte membrane antigens. Exp Cell Res 102: 9–13
Brecher G, Schneiderman M, Cronkite EP (1953) The reproducibility and constancy of the platelet count. Am J Clin Pathol 23: 15–26
Cutts JH, Krause WJ, Leeson CR (1980) Changes in the erythrocytes of the developing opossum, didelphis virginiana. Blood Cells 6: 55–62
Ebbe S, Stohlman F Jr, Donovan J, Howard D (1966) Platelet survival in the rat as measured with tritium-labeled diisopropylflourophosphate. J Lab Clin Med 68: 233–243
Fuhrmann GF, Ruhenstroth-Bauer G (1965) Cell elektrophoresis employing a rectangular measuring cuvette. In: Ambrose EJ (ed) Cell electrophoresis. Churchill, London, pp 22–25
Ganzoni AM (1970) Kinetik und Regulation der Erythrozytenproduktion. In: Experimentelle Medizin, Pathologie und Klinik, Bd 31. Springer, Berlin Heidelberg New York
Kachel V (1976) Basic principles of electrical sizing of cells and particles and their realisation in the new instrument “Metricell”. J Histochem Cytochem 24: 211–230
McDonald TP, Odell TT Jr, Gosslee DG (1964) Platelet size in relation to platelet age. Proc Soc Exp Biol Med 115: 684–689
Mitteilungen der IV. Conference on Automated Cytology (1976) Asilomar 1975. J Histochem Cytochem 24: 1–411
Odell TT Jr, Tausche FG, Furth J (1955) Platelet life span as measured by transfusion of isotopically labeled platelets into rats. Acta Haematol 13: 45–52
Schmidt G, Gross J, Grauel EL, Ihle W, Syllm-Rapoport I (1977) Separation and characterisation of red blood cells from newborns and infants during the first trimenon of life by means of a dextrane gradient. Biol Neonate 31: 42–50
Seaman GVF, Vassar PS (1966) Changes in the electrokinetic properties of platelets during their aggregation. Arch Biochem Biophys 117: 10–17
Tosteson DC (1966) Some properties of the plasma membranes of high potassium and low potassium sheep red cells. Ann NY Acad Sci [Art 2] 137: 577–590
Tucker EM (1975) Genetic markers in the plasma and red blood cells. In: Blunt MH (ed) The blood of sheep. Springer, Berlin Heidelberg New York, pp 123–153
Valet G, Franz G, Lauf PK (1978) Different red cell populations in newborn, genetically low potassium sheep: relation to hematopoietic, immunologie, and physiologie differentiation. J Cell Physiol 94: 215–227
Valet G, Hanser G, Ruhenstroth-Bauer G (1977) Different hemoglobins in different erythrocyte populations of the sheep and a new concept of the hematopoietic differentiation in the young mammalian organism. Exp Hematol [Suppl 2] 5: 79
Valet G, Hanser G, Ruhenstroth-Bauer G (1977) Ein neues Konzept der Hämatopoese im Säugetierorganismus während der Nachgeburtsphase: Nachweis mehrerer Volumenpopulationen der Erythrozyten bei Ratten, Mäusen, Meerschweinchen, Kaninchen, Schafen und Ziegen. Blut 34: 413–418
Valet G, Hofmann H, Ruhenstroth-Bauer G (1976) The computer analysis of volume distribution curves, demonstration of two erythrocyte populations of different size in the young guinea pig and analysis of the mechanism of the immune lysis of cells by antibody and complement. J Histochem Cytochem 24: 231–246
Valet G, Metzger H, Kachel V, Ruhenstroth-Bauer G (1972) Der Nachweis verschiedener Erythrozytenpopulationen bei der Ratte. Blut 24: 42–53
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Bamberger, S., Storch, F., Valet, G. et al. Postnatale Entwicklung von Rattenthrombozyten: Die Volumenverteilung und elektrophoretische Beweglichkeit von Rattenthrombozyten während des ersten Lebensmonats. Blut 40, 421–428 (1980). https://doi.org/10.1007/BF01029684
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01029684