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Blut

, Volume 40, Issue 1, pp 17–25 | Cite as

Proliferation activity and bacteriostatic potential of human blood monocytes, macrophages in pleural effusions, ascites, and of alveolar macrophages

  • Gerhard Meuret
  • Otto Schildknecht
  • Peter Joder
  • Hansjörg Senn
Original Works

Summary

Human blood monocytes, macrophages from pleural effusions, ascites, and alveolar macrophages obtained by bronchopulmonary lavage were investigated. The proliferative activity of these cells was determined by the3H-thymidine labeling index in vitro (3H-TDR L.I.). The bacteriostatic capacity was measured by the potential of the cells to block DNA-synthesis of proliferating Escherichia coli after phagocytosis.

In most cases3H-TDR L.I. of blood monocytes, macrophages from pleural effusions and ascites was less than 1 %. However, macrophages of some patients with neoplastic diseases exhibited3H-TDR L.I. between 4.0–9.6%. This probably reflected the action of factors, possibly lymphokines, which stimulated macrophage proliferation. In contrast, alveolar macrophages seemed to have almost totally lost their proliferative potential. The bacteriostatic capacity of blood monocytes proved to be significantly lower than that of macrophages. This demonstrates the functional immaturity of blood monocytes. In all types of macrophages investigated the bacteriostatic power was very high. No differences could be detected either between macrophages of different sources or between macrophages of benign, inflammatory, or malignant diseases.

Key words

Blood monocytes Macrophages Alveolar macrophages DNA-synthesis Bacteriostatic capacity 

Proliferationsaktivität und bakteriostatische Kapazität menschlicher Blutmonozyten, Makrophagen in Pleuraergüssen, Aszites und von Alveolarmakrophagen

Zusammenfassung

Untersucht wurden menschliche Blutmonozyten, Makrophagen von Pleuraergüssen, Aszites und Alveolarmakrophagen, die durch bronchopulmonale Lavage gewonnen wurden. Die Proliferationsaktivität dieser Zellen wurde durch die3H-Thymidin Inkorporation in vitro (3H-TDR L. I.) bestimmt. Die bakteriostatische Aktivität wurde gemessen aufgrund der Fähigkeit der Zellen die DNA-Synthese von Escherichia coli zu blockieren.

Bei der Mehrzahl der untersuchten Patienten lag der H-TDR L.I. der Blutmonozyten und der Ergußmakrophagen unter 1 %. Es wurden jedoch einige Fälle beobachtet, bei denen der3H-TDR L.I. der Makrophagen 4,0 bis 9,6 % erreichte. Dieser Beobachtung liegt möglicherweise die Einwirkung von Faktoren zugrunde, welche die Makrophagenproliferation stimulieren. Hierbei könnte es sich um Lymphokine handeln. Im Gegensatz zu den Ergußmakrophagen, besaßen die Alveolarmakrophagen praktisch keine Proliferationsfähigkeit mehr. Die bakteriostatische Kapazität der Blutmonozyten war erheblich geringer als diejenige der Makrophagen. Dies zeigt die relative Unreife der Blutmonozyten. Bei allen Arten untersuchter Makrophagen war das bakteriostatische Potential gleichermaßen außerordentlich hoch; krankheitsabhängige Unterschiede waren nicht erkennbar.

Schlüsselwörter

Blutmonozyten Makrophagen Alveolarmakrophagen DNS-Synthese bakteriostatische Kapazität 

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Copyright information

© Springer-Verlag 1980

Authors and Affiliations

  • Gerhard Meuret
    • 2
  • Otto Schildknecht
    • 1
  • Peter Joder
    • 1
  • Hansjörg Senn
    • 1
  1. 1.Abteilung für Hämatologie und OnkologieMedizinische Klinik CSt. GallenSwitzerland
  2. 2.Medizinische KlinikSt. Elisabethen-KrankenhausRavensburgGermany

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