Advertisement

Biotechnology Letters

, Volume 9, Issue 11, pp 751–756 | Cite as

Factors governing lactic acid formation in long term cultivation of hybridoma cells

  • Y. T. Luan
  • R. Mutharasan
  • W. E. Magee
Article

Summary

Effect of glucose concentration and pH on lactic acid formation was investigated in batch cultures of a hybridoma cell line. Lactate formation increases with growth rate. High glucose concentration leads to extensive lactate formation only during growth phase and not during stationary phase. Lactate formation also may serve to regulate extracellular pH to pH 6.8, provided conditions are favorable to maintain viability and if sufficient nutrients are present in the medium.

Keywords

Glucose Growth Rate Lactate Organic Chemistry Lactic Acid 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Glacken, M. W., Fleischaker, R. J., and Sinskey, A. J. (1986). Biotech. & Bioeng.,28, 1376–1389.Google Scholar
  2. Graff, S., Moser, H., Kastner, O., Graff, A. M. and Tannenbaum, M., (1965). J. Natl. Cancer Instit.,34, 511–519.Google Scholar
  3. Hatanaka, M., Todaro, G. J. and Gilden, R. V., (1970). Int. J. Cancer,5, 224–228.Google Scholar
  4. Hu, W. S., Dodge, T. C., Frame, K. K., and Himes, V. B. (1987). Dev. Biol. Standard.,66, 279–290.Google Scholar
  5. Lavery, M., Keams, M. J., Price, D. G., Emery, A. N., Jefferis, R., and Nienow, A. W. (1985). Dev. Biol. Standard.,60, 199–206.Google Scholar
  6. Luan, Y. T., Mutharasan, R., and Magee, W. E. (1987). Biotech. Lett., In press.Google Scholar
  7. Ozato, K., Mayer, N., and Schs, D.H. (1980). J. Immunol.124, 533–540.Google Scholar
  8. Renner, E. D. Plagemann, P. G. W. and Bernlohr, R. W., (1972). J. Biol. Chem.,247, 5765–5776.Google Scholar
  9. Reuveny, S., Velez, D., Riske, F., Macmillan J. D., and Miller, L. (1985). Dev. Biol. Standard.60, 185–197.Google Scholar
  10. van Wezel, A. L., van der Velden-de Groot, C. A. M., de Haan, H. H., van der Heuvel, N., and Schasfoort R. (1985). Dev. Biol. Standard.60, 229–236.Google Scholar
  11. Zielk, H. R., Ozand, P. T., Tilton, J. T., Sevdalian, D. A. and Cornblath, M., (1978). Cell Physiol.,95, 41–48.Google Scholar

Copyright information

© Kluwer Academic Publishers 1987

Authors and Affiliations

  • Y. T. Luan
    • 1
  • R. Mutharasan
    • 1
  • W. E. Magee
    • 2
  1. 1.Department of Chemical EngineeringDrexel UniversityPhiladelphiaU.S.A.
  2. 2.Department of Bioscience and BiotechnologyDrexel UniversityPhiladelphiaU.S.A.

Personalised recommendations