Biotechnology Letters

, Volume 8, Issue 8, pp 571–576 | Cite as

Rapid detection of microbial hydantoinase on solid medium

  • André Morin
  • Werner Hummel
  • Maria-Regina Kula
Article

Abstract

Hydantoin hydrolysing activity of microorganisms grown on solid complex media could be detected by an overlay assay with dihydrouracil as substrate. Positive strains developed a yellow colour around their colonies within 5 to 10 seconds after challenge with 10 uL drops of an acidic para-dimethylaminobenzaldehyde solution.

Keywords

Colour Organic Chemistry Bioorganic Chemistry Solid Medium Yellow Colour 

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References

  1. Cotoras, D. and Wagner, F. (1984). Stereospecific hydrolysis of 5-monosubstituted hydantoins. In Proc. 3rd Eur. Congr. Biotechnol. Vol. III. pp. 351–356. Munich. Verlag Chemie, Basel.Google Scholar
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Copyright information

© Kluwer Academic Publishers 1986

Authors and Affiliations

  • André Morin
    • 1
  • Werner Hummel
    • 1
    • 2
  • Maria-Regina Kula
    • 1
  1. 1.Gesellschaft für Biotechnologische Forschung mbHBraunschweig-StöckheimGermany
  2. 2.Institut für Enzymtechnologie der Universität Düsseldorf in der KFA JülichJülich 1Germany

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