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Interaction between hydrogen peroxide and ferrous sulfate as a basis for glucose determinations

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Summary

The interaction between hydrogen peroxide and ferrous sulfate (Fenton's reaction) results in an increase in absorbance between the wavelengths of 240–400 nm. The greatest increase in absorbance occurs at 260 nm. Addition of glucose to a solution of glucose oxidase and ferrous sulfate results in an instantaneous increase in absorbance at 260 nm which is dependent upon glucose concentration and pH, with greatest increase occurring at pH 4.0. A solution of glucose oxidase and ferrous sulfate could be used as a reagent for manual glucose determinations.

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Authors and Affiliations

  1. Chemical Technology Division, Oak Ridge National Laboratory, 37831, Oak Ridge, Tennessee

    Jonathan Woodward, Keith W. Lennon (Student), Gisella Zanin (Visiting scientist), Maria Wagner (Summer research participant) & Mary A. Scott

  2. Cooperative Education Program of the University of Tennessee, Tennessee, USA

    Keith W. Lennon (Student) & Mary A. Scott

  3. Department of Chemical Engineering, University of Maringa, Maringa, Brazil

    Gisella Zanin (Visiting scientist)

  4. Oak Ridge Associated Universities, Oak Ridge, USA

    Maria Wagner (Summer research participant)

Authors
  1. Jonathan Woodward
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  2. Keith W. Lennon
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  3. Gisella Zanin
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  4. Maria Wagner
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  5. Mary A. Scott
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Additional information

Operated by Martin Marietta Energy Systems, Inc., for the U.S. Department of Energy under Contract No. DE-ACO5-84OR21400.

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Woodward, J., Lennon, K.W., Zanin, G. et al. Interaction between hydrogen peroxide and ferrous sulfate as a basis for glucose determinations. Biotechnol Lett 7, 197–202 (1985). https://doi.org/10.1007/BF01027819

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  • DOI: https://doi.org/10.1007/BF01027819

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