Summary
A 2.4 Kb DNA fragment restricted from a Clarke-Carbon ColEl plasmid, pLC32-9, containing the xylose isomerase gene has been inserted into the PstI site of pDB248, a shuttle plasmid between the bacteriumE. coli and the fission yeast,Schizosaccharomyces pombe. This recombinant plasmid, pDB248-XI, can genetically complement xylose isomerase deficientE. coli strains and xylose isomerase gene can be expressed inSchizosaccharomyces pombe.
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Ueng, P.P., Volpp, K.J., Tucker, J.V. et al. Molecular cloning of theEscherichia coli gene encoding xylose isomerase. Biotechnol Lett 7, 153–158 (1985). https://doi.org/10.1007/BF01027809
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DOI: https://doi.org/10.1007/BF01027809