Summary
The consumption of macronutrients (carbon source, phosphate source, ratio of ammonium to nitrate, plant growth regulators) for biomass production in plant cell cultures ofSolanum tuberosum L, cv. Judith, are studied. This work is focussed on the improvement of the basal medium (Murashige and Skoog 1962) in order to decrease generation time of plant cells. The specific growth rate of plant cells. which grow on the modified medium is 0.41 day−1, compared to 0.19 day−1 on the Murashige and Skoog's medium.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Dougall, K. (1980), In “Plant tissue culture as a source of biochemicals”, Ed. by J. Staba. CRC Press, 21–28.
Gould, A.R., Evered, N.P., Wang, T.L., and Street, H.E. (1981), Protoplasma, 106: 1–13.
Henshaw, G.G., Jha, K.K., Mehta, A.R., Shakeshaft, D.J., and Street, H.E. (1966) J. Exp. Bot., 17: 362–377.
Murashige, T. and Skoog, F. (1962), Physiol. Plant. 15: 473–487.
Nakagawa, K., Konagai, A., Fukui, H., Tabata, T. (1984), Plant Cell Rep., 3: 254–257.
Takayama, S., Misawa, M., Kho K. and Misato, T. (1977), Physiol. Plant, 41: 313–320.
Waters and Salt (1980), AOAC Methods. Ed. by Horwitz W. 553–554.
Widholm, J.M. (1972), Stain Technol., 47, 189–194.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Gérad, J., Antoine, P., Lepoivre, P. et al. Uptake kinetics of medium constituents during batch growth of potato cell cultures. Biotechnol Lett 13, 381–384 (1991). https://doi.org/10.1007/BF01027687
Issue Date:
DOI: https://doi.org/10.1007/BF01027687