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Reduction of nucleic acid content in cell biomass of methanol-utilizing mixed bacterial culture by heat treatment

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Summary

A heat treatment method to reduce nucleic acid content in cell biomass of a mixed methanol-utilizing bacterial culture was studied. Maximum nucleic acid reduction in the bacterial cells was achieved by using heat shock at 65°C for 5–10 min followed by 2 h incubation at 55°C and 7.2±0.2 pH. In this treatment, 81–85% nucleic acid content was removed from the cells without affecting their true protein content and essential amino acids profile.

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Authors and Affiliations

  1. Kuwait Institute for Scientific Research, P. O. Box 24885, 13109, Safat, Kuwait

    A. S. Abu-Ruwaida, I. M. Banat & I. Y. Hamdan

Authors
  1. A. S. Abu-Ruwaida
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  2. I. M. Banat
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  3. I. Y. Hamdan
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Abu-Ruwaida, A.S., Banat, I.M. & Hamdan, I.Y. Reduction of nucleic acid content in cell biomass of methanol-utilizing mixed bacterial culture by heat treatment. Biotechnol Lett 10, 597–602 (1988). https://doi.org/10.1007/BF01027137

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  • DOI: https://doi.org/10.1007/BF01027137

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