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Effect of culture conditions on growth and sporulation ofBacillus thuringiensis subsp.israelensis and development of media for production of the protein crystal endotoxin

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Summary

The influence of medium composition on the inoculum and production stages of theBacillus thuringiensis subsp.israelensis bioinsecticide fermentation was investigated. Media which inhibited sporulation were selected for inoculum development stages. Bioinsecticide production media were designed to produce high cell counts and >90% sporulation in a 48h fermentation. Maximum insecticidal activity occurred at the point of maximum bacterial cell lysis/spore release. A process involving two inoculum stages and a 48h production stage in a 40 l fermenter yielded a viable cell count of 6.5 x 109/ml with greater than 95% sporulation. Good correlation existed between spore counts and bioinsecticide activity.

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Authors and Affiliations

  1. Delta Biotechnology, Castle Court, Castle Blvd, NG7 1FD, Nottingham, England

    D. Pearson

  2. Department of Biology, University of Waterloo, N2L 3G1, Waterloo, Ontario, Canada

    O. P. Ward

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  1. D. Pearson
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  2. O. P. Ward
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Pearson, D., Ward, O.P. Effect of culture conditions on growth and sporulation ofBacillus thuringiensis subsp.israelensis and development of media for production of the protein crystal endotoxin. Biotechnol Lett 10, 451–456 (1988). https://doi.org/10.1007/BF01027055

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  • DOI: https://doi.org/10.1007/BF01027055

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