Summary
Levansucrase (E.C. 2.4.1.10), which has long been known to be produced intracellularly byErwinia herbicola, was found in relatively high concentrations in the extracellular culture fluid of bacteria grown in various media. Sucrose was not required for enzyme secretion. A medium consisting of corn steep liquor and sorbitol or mannitol gave the highest yields of enzyme.
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Cote, G.L. Production of a constitutive, extracellular levansucrase fromErwinia herbicola NRRL B-1678. Biotechnol Lett 10, 879–882 (1988). https://doi.org/10.1007/BF01026999
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DOI: https://doi.org/10.1007/BF01026999