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Fates and expression ofBacillus subtilis endo-β-1, 4-glucanase gene andAlcaligenes faecalis β-glucosidase gene introduced inEscherichia coli andBacillus cells

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Summary

Two kinds of cellulase genes coding for endo-β-1, 4-glucanase and β-glucosidase, isolated fromBacillus subtilis andAlcaligenes faecalis respectively, were separately or combinedly put on a newly constructedEscherichia coli-Bacillus shuttle vector plasmid. When the recombinant plasmids having cellulase gene(s) were introduced intoE. coli orBacillus cells, drastic differences in fates and expression of the two genes were observed.

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Authors and Affiliations

  1. Department of Food and Nutrition, Inje University, 621-749, Kimhae, South Korea

    Dong Seok Lee

  2. Department of Biological Science and Engineering, Korea Advanced Institute of Science and Technology, Cheongryang, P. O. Box 150, Seoul, South Korea

    B. R. Lee & M. Y. Pack

Authors
  1. Dong Seok Lee
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  2. B. R. Lee
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  3. M. Y. Pack
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Lee, D.S., Lee, B.R. & Pack, M.Y. Fates and expression ofBacillus subtilis endo-β-1, 4-glucanase gene andAlcaligenes faecalis β-glucosidase gene introduced inEscherichia coli andBacillus cells. Biotechnol Lett 10, 843–848 (1988). https://doi.org/10.1007/BF01026993

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  • DOI: https://doi.org/10.1007/BF01026993

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